mirror of
https://github.com/chanzuckerberg/cellxgene.git
synced 2026-10-05 03:48:12 +08:00
Merge branch 'master' of https://github.com/chanzuckerberg/cellxgene into bkmartinjr-crossfiltergroups
This commit is contained in:
@@ -41,7 +41,8 @@ Started in the context of the Human Cell Atlas Consortium, cellxgene hopes to bo
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**Install server**
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**Install server**
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pip install -e .
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pip install -e .
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**Run (with demo data)**
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**Run (with demo data)**
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@@ -56,6 +57,72 @@ _For help with the scanpy engine_
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cellxgene scanpy --help
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cellxgene scanpy --help
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## Using your own data
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### Scanpy
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To prepare your data you will need to format your data into AnnData format using scanpy and calculate PCA and nearest neighbors and save in h5ad format.
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1. [Load data into scanpy](https://scanpy.readthedocs.io/en/latest/api/index.html#reading)
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- Ensure that `obs`'s index is the cell names: `print(data.obs_names)` should show your cell indices. If it shows gene names, you may need to just call `data.transpose()`.
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2. Calculate PCA
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sc.pp.pca(data) ## sc is scanpy.api
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3. Calculate nearest neighbors (depending on layout algorithm)
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```
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# For umap layout algorithm, you need to use the "umap" method for neighbors
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sc.pp.neighbors(data, method="umap", metric="euclidean", use_rep="X_pca")
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# For tsne layout algorithm, you can use either "umap" or "gauss"; we recommend "gauss"
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sc.pp.neighbors(data, method="gauss", metric="euclidean", use_rep="X_pca")
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```
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4. Save file
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```
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# cellxgene requires file to be named data.h5ad
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data.write("data.h5ad")
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```
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5. Create config file (optional)
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If you do not have a config file, the schema (metadata names, types, and categorical/continuous) will be inferred from the observations in the data file. Config file is required to be named 'data_schema.json' and located in the same directory as data file.
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- The config file is a JSON format file with information on the metadata associated with the cells. The key is the column name in obs. The value is an object
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```
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type: string, int, or float (what type the values are),
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variabletype: categorical or continuous (categorical values are displayed as checkboxes, continuous values are displayed as a histogram)
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displayname: (what the heading should be displayed as)
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include: True/False (whether to display values on web interface)
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```
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```
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Example
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{
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"CellName": {
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"type": "string",
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"variabletype": "categorical",
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"displayname": "Name",
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"include": true
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},
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"clusters": {
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"type": "string",
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"variabletype": "categorical",
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"displayname": "Clusters",
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"include": true
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},
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"num_genes": {
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"type": "int",
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"variabletype": "continuous",
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"displayname": "Number Genes",
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"include": true
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}
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}
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```
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## Contributing
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## Contributing
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We warmly welcome contributions from the community. Please submit any bug reports and feature requests through github issues. Please submit any direct contributions via a branch + pull request.
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We warmly welcome contributions from the community. Please submit any bug reports and feature requests through github issues. Please submit any direct contributions via a branch + pull request.
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+5
-7
@@ -2,6 +2,7 @@
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"name": "cellxgene",
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"name": "cellxgene",
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"version": "0.0.1",
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"version": "0.0.1",
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"license": "MIT",
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"license": "MIT",
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"description": "cellxgene is a web application for the interactive exploration of single cell sequence data.",
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"repository": "https://github.com/chanzuckerberg/cellxgene",
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"repository": "https://github.com/chanzuckerberg/cellxgene",
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"scripts": {
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"scripts": {
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"build": "npm run clean && webpack --config configuration/webpack/webpack.config.prod.js",
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"build": "npm run clean && webpack --config configuration/webpack/webpack.config.prod.js",
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@@ -29,16 +30,13 @@
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"dependencies": {
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"dependencies": {
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"canvas-fit": "^1.5.0",
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"canvas-fit": "^1.5.0",
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"d3": "^4.10.0",
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"d3": "^4.10.0",
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"deck.gl": "^4.1.2",
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"express": "^4.14.0",
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"express": "^4.14.0",
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"font-color-contrast": "^1.0.3",
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"font-color-contrast": "^1.0.3",
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"gl-mat4": "^1.1.4",
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"gl-mat4": "^1.1.4",
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"gl-matrix": "^2.7.1",
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"gl-matrix": "^2.7.1",
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"halogen": "^0.2.0",
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"hsv2rgb": "^1.1.0",
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"hsv2rgb": "^1.1.0",
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"key-pressed": "0.0.1",
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"key-pressed": "0.0.1",
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"lodash": "^4.17.4",
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"lodash": "^4.17.4",
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"luma.gl": "^4.0.3",
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"mouse-position": "^2.0.1",
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"mouse-position": "^2.0.1",
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"mouse-pressed": "^1.0.0",
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"mouse-pressed": "^1.0.0",
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"orbit-camera": "^1.0.0",
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"orbit-camera": "^1.0.0",
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@@ -50,7 +48,6 @@
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"react-hot-loader": "^3.0.0-beta.7",
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"react-hot-loader": "^3.0.0-beta.7",
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"react-icons": "^2.2.7",
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"react-icons": "^2.2.7",
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"react-redux": "^5.0.6",
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"react-redux": "^5.0.6",
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"react-router-dom": "4.1.2",
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"redux": "^3.7.2",
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"redux": "^3.7.2",
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"redux-thunk": "^2.2.0",
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"redux-thunk": "^2.2.0",
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"regl": "^1.3.1",
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"regl": "^1.3.1",
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@@ -81,12 +78,12 @@
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"cross-env": "^3.1.4",
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"cross-env": "^3.1.4",
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"css-loader": "^0.26.1",
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"css-loader": "^0.26.1",
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"css-modules-require-hook": "^4.0.1",
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"css-modules-require-hook": "^4.0.1",
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"enzyme": "^2.4.1",
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"enzyme": "^3.3.0",
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"eslint": "^4.18.2",
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"eslint": "^4.18.2",
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"eslint-loader": "^1.5.0",
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"eslint-loader": "^1.5.0",
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"eslint-plugin-filenames": "^1.1.0",
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"eslint-plugin-filenames": "^1.1.0",
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"eslint-plugin-import": "^2.2.0",
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"eslint-plugin-import": "^2.2.0",
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"eslint-plugin-jsx-a11y": "^3.0.2",
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"eslint-plugin-jsx-a11y": "^6.1.1",
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"eslint-plugin-react": "^6.0.0",
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"eslint-plugin-react": "^6.0.0",
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"extract-text-webpack-plugin": "^2.0.0-beta.3",
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"extract-text-webpack-plugin": "^2.0.0-beta.3",
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"file-loader": "^0.9.0",
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"file-loader": "^0.9.0",
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"jest": "^23.4.1",
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"jest": "^23.4.1",
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"jsdom": "^9.4.1",
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"jsdom": "^9.4.1",
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"json-loader": "^0.5.4",
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"json-loader": "^0.5.4",
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"nyc": "^10.0.0",
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"nyc": "^13.0.1",
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"postcss": "^6.0.0",
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"postcss-loader": "^1.2.2",
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"postcss-loader": "^1.2.2",
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"promise": "^7.1.1",
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"promise": "^7.1.1",
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"react-addons-test-utils": "^15.3.0",
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"react-addons-test-utils": "^15.3.0",
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