mirror of
https://github.com/lh3/minimap2.git
synced 2026-09-15 13:07:55 +08:00
r718: retrieve sequence from the index
This commit is contained in:
@@ -34,6 +34,8 @@ The following Python script demonstrates the key functionality of mappy:
|
||||
import mappy as mp
|
||||
a = mp.Aligner("test/MT-human.fa") # load or build index
|
||||
if not a: raise Exception("ERROR: failed to load/build index")
|
||||
s = a.seq("MT_human", 100, 200) # retrieve a subsequence from the index
|
||||
print(mp.revcomp(s)) # reverse complement
|
||||
for name, seq, qual in mp.fastx_read("test/MT-orang.fa"): # read a fasta/q sequence
|
||||
for hit in a.map(seq): # traverse alignments
|
||||
print("{}\t{}\t{}\t{}".format(hit.ctg, hit.r_st, hit.r_en, hit.cigar_str))
|
||||
@@ -87,7 +89,15 @@ This method aligns :code:`seq` against the index. It is a generator, *yielding*
|
||||
a series of :code:`mappy.Alignment` objects. If :code:`seq2` is present, mappy
|
||||
performs paired-end alignment, assuming the two ends are in the FR orientation.
|
||||
Alignments of the two ends can be distinguished by the :code:`read_num` field
|
||||
(see below).
|
||||
(see Class mappy.Alignment below).
|
||||
|
||||
.. code:: python
|
||||
|
||||
mappy.Aligner.seq(name, start=0, end=0x7fffffff)
|
||||
|
||||
This method retrieves a (sub)sequence from the index and returns it as a Python
|
||||
string. :code:`None` is returned if :code:`name` is not present in the index or
|
||||
the start/end coordinates are invalid.
|
||||
|
||||
Class mappy.Alignment
|
||||
~~~~~~~~~~~~~~~~~~~~~
|
||||
@@ -144,11 +154,12 @@ Miscellaneous Functions
|
||||
|
||||
.. code:: python
|
||||
|
||||
mappy.fastx_read(fn)
|
||||
mappy.fastx_read(fn, read_comment=False)
|
||||
|
||||
This generator function opens a FASTA/FASTQ file and *yields* a
|
||||
:code:`(name,seq,qual)` tuple for each sequence entry. The input file may be
|
||||
optionally gzip'd.
|
||||
optionally gzip'd. If :code:`read_comment` is True, this generator yields
|
||||
a :code:`(name,seq,qual,comment)` tuple instead.
|
||||
|
||||
.. code:: python
|
||||
|
||||
|
||||
Reference in New Issue
Block a user