From 3d351267a0264d8a6e2a6619a40fe3bb3716701a Mon Sep 17 00:00:00 2001 From: Heng Li Date: Sun, 6 Apr 2025 17:43:11 -0400 Subject: [PATCH] document --jump-bed --- minimap2.1 | 22 ++++++++++++++++------ 1 file changed, 16 insertions(+), 6 deletions(-) diff --git a/minimap2.1 b/minimap2.1 index a0f8ab9..2f9aed9 100644 --- a/minimap2.1 +++ b/minimap2.1 @@ -1,4 +1,4 @@ -.TH minimap2 1 "2 April 2025" "minimap2-2.28-dirty (r1248)" "Bioinformatics tools" +.TH minimap2 1 "6 April 2025" "minimap2-2.28-dirty (r1259)" "Bioinformatics tools" .SH NAME .PP minimap2 - mapping and alignment between collections of DNA sequences @@ -418,7 +418,7 @@ Penalty of a mismatch involving ambiguous bases [1]. .TP .BI --pe-ind-chain For paired-end short reads, perform chaining for each end independently. -By default, minimap2 chains the two ends together. +By default, minimap2 jointly chains the two ends. .TP .BR --splice-flank = yes | no Assume the next base to a @@ -453,10 +453,13 @@ but not .BR --junc-bed . .TP .BR --junc-bed \ FILE -Gene annotations in the BED12 format (aka 12-column BED), or intron positions -in 5-column BED. With this option, minimap2 prefers splicing in annotations. -BED12 file can be converted from GTF/GFF3 with `paftools.js gff2bed anno.gtf' -[]. +Junctions to prefer during base alignment. +.I FILE +can be gene annotations in the BED12 format (aka 12-column BED), or intron +positions in 5-column BED. BED12 file can be converted from GTF/GFF3 with +`paftools.js gff2bed anno.gtf'. It is +.I NOT +recommended to apply this option for short RNA-seq reads. [] .TP .BR --junc-bonus \ INT Score bonus for a splice donor or acceptor found in annotation [9]. Effective with @@ -464,6 +467,13 @@ Score bonus for a splice donor or acceptor found in annotation [9]. Effective wi but not .BR --spsc . .TP +.BR --jump-bed \ FILE +Junctions used to extend alignment towards ends of reads. Same format as with +.BR --junc-bed . +This option is intended for short RNA-seq reads, while +.B --junc-bed +for long noisy RNA-seq reads. [] +.TP .BI --end-seed-pen \ INT Drop a terminal anchor if .IR s