diff --git a/python/mappy.pyx b/python/mappy.pyx index d236a1a..7d412fe 100644 --- a/python/mappy.pyx +++ b/python/mappy.pyx @@ -189,7 +189,6 @@ cdef class Aligner: if self._idx is NULL: return None if buf is None: b = ThreadBuffer() else: b = buf - km = cmappy.mm_tbuf_get_km(b._b) _seq = seq if isinstance(seq, bytes) else seq.encode() if name is not None: @@ -216,6 +215,7 @@ cdef class Aligner: c = h.cigar32[k] cigar.append([c>>4, c&0xf]) if cs or ds or MD: # generate the cs/ds and/or the MD tag, if requested + km = cmappy.mm_tbuf_get_km(b._b) _cur_seq = _seq2 if h.seg_id > 0 and seq2 is not None else _seq if cs: l_cs_str = cmappy.mm_gen_cs(km, &cs_str, &m_cs_str, self._idx, ®s[i], _cur_seq, 1)