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In human/mouse, the GTr..yAG pattern occurs to 91/92% of all GT-AG introns. Modeling r..y clearly leads to higher accuracy. However, in SIRV, this percentage is reduced to ~60%. The default "--splice --splice-flank=yes" leads to lower accuracy. If someone benchmark minimap2 on SIRV, this would be bad, but minimap2 is developed for practical applications, not for benchmarks. I will live with that.
20 KiB
20 KiB