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In the default splice mode, minimap2 applies two rounds of spliced alignment: first assuming GT-AG to be the splice signal across all splicing sites and then assuming CT-AC to be the signal. This is the idea strategy. In the MM_F_SPLICE_BOTH mode, minimap2 applies one round of spliced alignment, assuming GT-AG and CT-AC to be the splice signals AT THE SAME TIME. This will be faster but less accurate. I don't think anyone would like to run minimap2 in this mode, so I am removing it for clarity.
17 KiB
17 KiB