diff --git a/chart/charts.py b/chart/charts.py
index 102c9a5..e86ffdf 100644
--- a/chart/charts.py
+++ b/chart/charts.py
@@ -3861,8 +3861,7 @@ def Aggregated_Pyramids(file_path, char_out_path, out_path, title, caption, subt
         """
 
     keep_txt_to_file(code, f"{out_path}.R")
-    os.system(
-        f"Rscript {out_path}.R")
+    os.system(f"Rscript {out_path}.R")
 
 
 # 基础参数 file_path, char_out_path, out_path, title, caption, subtitle, title_font_size, x_name, x_font_size, x_scale_font_size, y_name, y_font_size, y_scale_font_size, event_1, event_2, event_3, font_size
@@ -3989,8 +3988,7 @@ def Areas_Under_a_Time_Series(file_path, char_out_path, out_path, title, caption
         """
 
     keep_txt_to_file(code, f"{out_path}.R")
-    os.system(
-        f"Rscript {out_path}.R")
+    os.system(f"Rscript {out_path}.R")
 
 
 # 基础参数 file_path, char_out_path, out_path, title, caption_1, caption_2, subtitle, title_font_size, font_size
@@ -4085,8 +4083,8 @@ def Balloon_Plot(file_path, char_out_path, out_path, title, caption_1, caption_2
         """
 
     keep_txt_to_file(code, f"{out_path}.R")
-    os.system(
-        f"Rscript {out_path}.R")  # 基础参数 file_path, char_out_path, out_path, title, caption, subtitle, title_font_size, x_name, x_font_size, x_scale_font_size, y_name, y_font_size, y_scale_font_size, image_name_font_size, mark_country_1, mark_country_2, average_font_size
+    os.system(f"Rscript {out_path}.R")  # 基础参数 file_path, char_out_path, out_path, title, caption, subtitle, title_font_size, x_name, x_font_size, x_scale_font_size, y_name, y_font_size, y_scale_font_size, image_name_font_size, mark_country_1, mark_country_2, average_font_size
+
 
 
 # 高级参数 title_font_color, x_font_color, x_scale_font_color, y_font_color, y_scale_font_color, image_name_font_color, average_font_color, mark_color, color
diff --git a/static/css/layui.css b/static/css/layui.css
index 3d73a90..2cc0e95 100644
--- a/static/css/layui.css
+++ b/static/css/layui.css
@@ -3951,10 +3951,6 @@ body .layui-table-tips .layui-layer-content {
     font-size: 14px;  // 所有字体大小
 }
 
-.layui-nav-head * {
-    font-size: 14px;  // 所有字体大小
-}
-
 .layui-nav .layui-nav-item {
     position: relative;
     display: inline-block;
diff --git a/templates/chart/Basic.html b/templates/chart/Basic.html
index 8cc9d20..159b7ae 100644
--- a/templates/chart/Basic.html
+++ b/templates/chart/Basic.html
@@ -15,11 +15,11 @@
         <div class="layui-logo layui-hide-xs layui-bg-black">Simple Drawing</div>
         <ul class="layui-nav layui-layout-left">
 
-            <li class="layui-nav-item layui-hide-xs layui-this" style="margin-left: 200px"><a href="{{ url }}">Home</a>
+            <li class="layui-nav-item layui-hide-xs layui-this" style="margin-left: 200px"><a style="font-size: 30px;" href="{{ url }}">Home</a>
             </li>
-            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a
+            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a style="font-size: 30px;"
                     href="tools.html">Tools</a></li>
-            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a href="about.html">About
+            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a style="font-size: 30px;" href="about.html">About
                 us</a></li>
 
             <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><i
@@ -51,22 +51,22 @@
                     </button>
                 </li>
 
-                <li class="layui-nav-item">
-                    <a href="javascript:">Basic settings</a>
+                <li class="layui-nav-item" style="margin-top: 20px;">
+                    <a href="javascript:" style="font-size: 20px;">Basic settings</a>
                     <dl class="layui-nav-child">
                         {{ Basics }}
                     </dl>
                 </li>
 
-                <li class="layui-nav-item">
-                    <a href="javascript:">Advanced setting</a>
+                <li class="layui-nav-item" style="margin-top: 10px;">
+                    <a href="javascript:" style="font-size: 20px;">Advanced setting</a>
                     <dl class="layui-nav-child">
                         {{ Senior }}
                     </dl>
                 </li>
 
-                <li class="layui-nav-item">
-                    <a href="javascript:">downlode</a>
+                <li class="layui-nav-item" style="margin-top: 10px;">
+                    <a href="javascript:" style="font-size: 20px;">downlode</a>
                     <dl class="layui-nav-child">
 
                         <dd>
@@ -85,7 +85,7 @@
 
                         <dd style="text-align: center">
                             <button type="button" class="layui-btn layui-btn-radius">
-                                <i class="layui-icon layui-icon-download-circle"
+                                <i class="layui-icon layui-icon-download-circle" style="margin-top: 10px;"
                                    style="width: 100px;font-size: 28px;left: 5px" onclick=down_file()></i>
                             </button>
                         </dd>
@@ -93,7 +93,7 @@
                     </dl>
                 </li>
 
-                <li class="layui-nav-item NO "><a href={{ url }}/chart/down?name={{ example_file }}>download eg file</a>
+                <li class="layui-nav-item NO "><a href={{ url }}/chart/down?name={{ example_file }} style="font-size: 20px;">download eg file</a>
                 </li>
 
             </ul>
diff --git a/templates/page/about.html b/templates/page/about.html
index 2f51620..e3d56fe 100644
--- a/templates/page/about.html
+++ b/templates/page/about.html
@@ -18,10 +18,10 @@
         <div class="layui-logo layui-hide-xs layui-bg-black">Simple Drawing</div>
         <ul class="layui-nav layui-layout-left">
 
-            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a href="{{ url }}">Home</a></li>
-            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a
+            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a style="font-size: 30px;" href="{{ url }}">Home</a></li>
+            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a style="font-size: 30px;"
                     href="tools.html">Tools</a></li>
-            <li class="layui-nav-item layui-hide-xs layui-this" style="margin-left: 200px"><a href="about.html">About
+            <li class="layui-nav-item layui-hide-xs layui-this" style="margin-left: 200px"><a style="font-size: 30px;" href="about.html">About
                 us</a></li>
 
             <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><i
@@ -46,21 +46,21 @@
         <div class="layui-side-scroll">
             <!-- 左侧导航区域（可配合layui已有的垂直导航） -->
             <ul class="layui-nav layui-nav-tree" lay-filter="test">
-                <li class="layui-nav-item layui-hide-xs" onclick="select_1()" id="brief_introduction"><a>brief
+                <li class="layui-nav-item layui-hide-xs" style="margin-top: 20px;" onclick="select_1()" id="brief_introduction"><a style="font-size: 25px;">brief
                     introduction</a>
                 </li>
                 <hr class="layui-border-black">
 
-                <li class="layui-nav-item layui-hide-xs" onclick="select_2()" id="Leaving_a_message"><a>Leaving
+                <li class="layui-nav-item layui-hide-xs" style="margin-top: 10px;" onclick="select_2()" id="Leaving_a_message"><a style="font-size: 25px;">Leaving
                     a message</a>
                 </li>
                 <hr class="layui-border-black">
 
-                <li class="layui-nav-item layui-hide-xs" onclick="select_3()" id="Declare"><a>Declare</a>
+                <li class="layui-nav-item layui-hide-xs" style="margin-top: 10px;" onclick="select_3()" id="Declare"><a style="font-size: 25px;">Declare</a>
                 </li>
                 <hr class="layui-border-black">
 
-                <li class="layui-nav-item layui-hide-xs" onclick="select_4()" id="Timeline"><a>Timeline</a>
+                <li class="layui-nav-item layui-hide-xs" style="margin-top: 10px;" onclick="select_4()" id="Timeline"><a style="font-size: 25px;">Timeline</a>
                 </li>
 
 
diff --git a/templates/page/index.html b/templates/page/index.html
index 7ba575a..f524e69 100644
--- a/templates/page/index.html
+++ b/templates/page/index.html
@@ -19,12 +19,12 @@
 
                 <ul class="layui-nav layui-layout-left">
 
-                    <li class="layui-nav-item layui-hide-xs layui-this" style="margin-left: 200px"><a
+                    <li class="layui-nav-item layui-hide-xs layui-this" style="margin-left: 200px"><a style="font-size: 30px;"
                             href="index.html">Home</a></li>
 
-                    <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a href="tools.html">Tools</a></li>
+                    <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a style="font-size: 30px;" href="tools.html">Tools</a></li>
 
-                    <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a href="about.html">About us</a></li>
+                    <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a style="font-size: 30px;" href="about.html">About us</a></li>
 
                     <li class="layui-nav-item layui-hide-xs" style="margin-left: 150px"><i class="layui-icon layui-icon-search"></i></li>
 
@@ -39,14 +39,14 @@
     <div class="layui-side layui-bg-black">
         <div class="layui-side-scroll">
             <ul class="layui-nav layui-nav-tree" lay-filter="test">
-                <li class="layui-nav-item NO "><a onclick=ALL()>ALL</a></li>
-                <li class="layui-nav-item NO "><a onclick=Scatter_plot()>Scatter plot</a></li>
-                <li class="layui-nav-item NO "><a onclick=Line_chart()>Line chart</a></li>
-                <li class="layui-nav-item NO "><a onclick=Pie_chart()>Pie chart</a></li>
-                <li class="layui-nav-item NO "><a onclick=Histogram()>Histogram</a></li>
-                <li class="layui-nav-item NO "><a onclick=Heat_map()>Heat map</a></li>
-                <li class="layui-nav-item NO "><a onclick=Dot_plot()>Dot plot</a></li>
-                <li class="layui-nav-item NO "><a onclick=Chord_Diagram()>Chord Diagram</a></li>
+                <li style="margin-top: 20px;" class="layui-nav-item NO "><a style="font-size: 25px;" onclick=ALL()>ALL</a></li>
+                <li style="margin-top: 10px;" class="layui-nav-item NO "><a style="font-size: 25px;" onclick=Scatter_plot()>Scatter plot</a></li>
+                <li style="margin-top: 10px;" class="layui-nav-item NO "><a style="font-size: 25px;" onclick=Line_chart()>Line chart</a></li>
+                <li style="margin-top: 10px;" class="layui-nav-item NO "><a style="font-size: 25px;" onclick=Pie_chart()>Pie chart</a></li>
+                <li style="margin-top: 10px;" class="layui-nav-item NO "><a style="font-size: 25px;" onclick=Histogram()>Histogram</a></li>
+                <li style="margin-top: 10px;" class="layui-nav-item NO "><a style="font-size: 25px;" onclick=Heat_map()>Heat map</a></li>
+                <li style="margin-top: 10px;" class="layui-nav-item NO "><a style="font-size: 25px;" onclick=Dot_plot()>Dot plot</a></li>
+                <li style="margin-top: 10px;" class="layui-nav-item NO "><a style="font-size: 25px;" onclick=Chord_Diagram()>Chord Diagram</a></li>
 
             </ul>
         </div>
diff --git a/templates/page/tools.html b/templates/page/tools.html
index e3340c6..80d2c73 100644
--- a/templates/page/tools.html
+++ b/templates/page/tools.html
@@ -17,10 +17,10 @@
         <!-- 头部区域（可配合layui 已有的水平导航） -->
         <ul class="layui-nav layui-layout-left">
 
-            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a href="index.html">Home</a></li>
-            <li class="layui-nav-item layui-hide-xs layui-this" style="margin-left: 200px"><a
+            <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><a style="font-size: 30px;" href="index.html">Home</a></li>
+            <li class="layui-nav-item layui-hide-xs layui-this" style="margin-left: 200px"><a style="font-size: 30px;"
                     href="tools.html">Tools</a></li>
-            <li class="layui-nav-item layui-hide-xs " style="margin-left: 200px"><a href="about.html">About us</a></li>
+            <li class="layui-nav-item layui-hide-xs " style="margin-left: 200px"><a style="font-size: 30px;" href="about.html">About us</a></li>
 
             <li class="layui-nav-item layui-hide-xs" style="margin-left: 200px"><i
                     class="layui-icon layui-icon-search"></i></li>
@@ -38,8 +38,8 @@
         <div class="layui-side-scroll">
             <!-- 左侧导航区域（可配合layui已有的垂直导航） -->
             <ul class="layui-nav layui-nav-tree" lay-filter="test">
-                <li class="layui-nav-item">
-                    <a class="" href="javascript:;">Basics</a>
+                <li style="margin-top: 20px;" class="layui-nav-item">
+                    <a style="font-size: 25px;" class="" href="javascript:;">Basics</a>
                     <dl class="layui-nav-child">
                         <dd><a onclick=blast()>blast</a></dd>
                         <dd><a onclick="genbank_to_fasta()">genbank to fasta</a></dd>
@@ -49,14 +49,14 @@
 
                     </dl>
                 </li>
-                <li class="layui-nav-item">
-                    <a href="javascript:;">Gene Family</a>
+                <li style="margin-top: 10px;" class="layui-nav-item">
+                    <a style="font-size: 25px;" href="javascript:;">Gene Family</a>
                     <dl class="layui-nav-child">
                         <dd><a onclick=pfam()>pfam</a></dd>
                     </dl>
                 </li>
-                <li class="layui-nav-item">
-                    <a href="javascript:;">Omics</a>
+                <li style="margin-top: 10px;" class="layui-nav-item">
+                    <a style="font-size: 25px;" href="javascript:;">Omics</a>
                     <dl class="layui-nav-child">
                         <dd><a href="javascript:;" onclick=Differential_expression_analysis()>Differential expression
                             analysis</a></dd>