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update coverage

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chhylp123
2020-07-13 22:34:26 -04:00
parent c01b593d7c
commit fda858f9e7
8 changed files with 449 additions and 104 deletions
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87
CommandLines.cpp
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@@ -19,6 +19,8 @@ static ko_longopt_t long_options[] = {
{ "max-od-final", ko_no_argument, 306 },
{ "ex-list", ko_required_argument, 307 },
{ "ex-iter", ko_required_argument, 308 },
{ "purge-cov", ko_required_argument, 309 },
{ "pri-range", ko_required_argument, 310 },
{ 0, 0, 0 }
};
@@ -34,41 +36,45 @@ void Print_H(hifiasm_opt_t* asm_opt)
fprintf(stderr, "Usage: hifiasm [options] <in_1.fq> <in_2.fq> <...>\n");
fprintf(stderr, "Options:\n");
fprintf(stderr, " Assembly:\n");
fprintf(stderr, " -o FILE prefix of output files [%s]\n", asm_opt->output_file_name);
fprintf(stderr, " -t INT number of threads [%d]\n", asm_opt->thread_num);
fprintf(stderr, " -r INT round of correction [%d]\n", asm_opt->number_of_round);
fprintf(stderr, " -a INT round of assembly cleaning [%d]\n", asm_opt->clean_round);
fprintf(stderr, " -k INT k-mer length (must be <64) [%d]\n", asm_opt->k_mer_length);
fprintf(stderr, " -w INT minimizer window size [%d]\n", asm_opt->mz_win);
fprintf(stderr, " -f INT number of bits for bloom filter; 0 to disable [%d]\n", asm_opt->bf_shift);
fprintf(stderr, " -D FLOAT drop k-mers occuring >FLOAT*coverage times [%.1f]\n", asm_opt->high_factor);
fprintf(stderr, " -N INT consider up to max(-D*coverage,-N) overlaps for each oriented read [%d]\n", asm_opt->max_n_chain);
fprintf(stderr, " -i ignore saved overlaps in *.ovlp* files\n");
fprintf(stderr, " -z INT length of adapters that should be removed [%d]\n", asm_opt->adapterLen);
fprintf(stderr, " -m INT size of popped large bubbles for contig graph [%lld]\n", asm_opt->large_pop_bubble_size);
fprintf(stderr, " -p INT size of popped small bubbles for haplotype-resolved unitig graph [%lld]\n", asm_opt->small_pop_bubble_size);
fprintf(stderr, " -n INT small removed unitig threshold [%d]\n", asm_opt->max_short_tip);
fprintf(stderr, " -x FLOAT max overlap drop ratio [%.2g]\n", asm_opt->max_drop_rate);
fprintf(stderr, " -y FLOAT min overlap drop ratio [%.2g]\n", asm_opt->min_drop_rate);
fprintf(stderr, " -u disable post join contigs step which may improve N50. Don't disable in default.\n");
fprintf(stderr, " --version show version number\n");
fprintf(stderr, " -h show help information\n");
fprintf(stderr, " -o FILE prefix of output files [%s]\n", asm_opt->output_file_name);
fprintf(stderr, " -t INT number of threads [%d]\n", asm_opt->thread_num);
fprintf(stderr, " -r INT round of correction [%d]\n", asm_opt->number_of_round);
fprintf(stderr, " -a INT round of assembly cleaning [%d]\n", asm_opt->clean_round);
fprintf(stderr, " -k INT k-mer length (must be <64) [%d]\n", asm_opt->k_mer_length);
fprintf(stderr, " -w INT minimizer window size [%d]\n", asm_opt->mz_win);
fprintf(stderr, " -f INT number of bits for bloom filter; 0 to disable [%d]\n", asm_opt->bf_shift);
fprintf(stderr, " -D FLOAT drop k-mers occuring >FLOAT*coverage times [%.1f]\n", asm_opt->high_factor);
fprintf(stderr, " -N INT consider up to max(-D*coverage,-N) overlaps for each oriented read [%d]\n", asm_opt->max_n_chain);
fprintf(stderr, " -i ignore saved overlaps in *.ovlp* files\n");
fprintf(stderr, " -z INT length of adapters that should be removed [%d]\n", asm_opt->adapterLen);
fprintf(stderr, " -m INT size of popped large bubbles for contig graph [%lld]\n", asm_opt->large_pop_bubble_size);
fprintf(stderr, " -p INT size of popped small bubbles for haplotype-resolved unitig graph [%lld]\n", asm_opt->small_pop_bubble_size);
fprintf(stderr, " -n INT small removed unitig threshold [%d]\n", asm_opt->max_short_tip);
fprintf(stderr, " -x FLOAT max overlap drop ratio [%.2g]\n", asm_opt->max_drop_rate);
fprintf(stderr, " -y FLOAT min overlap drop ratio [%.2g]\n", asm_opt->min_drop_rate);
fprintf(stderr, " --pri-range INT,INT keep contigs with coverage in this range at p_ctg.gfa. Inferred automatically in default. Set -1,-1 to disable\n");
fprintf(stderr, " -u disable post join contigs step which may improve N50. Don't disable in default.\n");
fprintf(stderr, " --version show version number\n");
fprintf(stderr, " -h show help information\n");
fprintf(stderr, " Trio-partition:\n");
fprintf(stderr, " -1 FILE hap1/paternal k-mer dump generated by \"yak count\" []\n");
fprintf(stderr, " -2 FILE hap2/maternal k-mer dump generated by \"yak count\" []\n");
fprintf(stderr, " -3 FILE list of hap1/paternal read names []\n");
fprintf(stderr, " -4 FILE list of hap2/maternal read names []\n");
fprintf(stderr, " -c INT lower bound of the binned k-mer's frequency [%d]\n", asm_opt->min_cnt);
fprintf(stderr, " -d INT upper bound of the binned k-mer's frequency [%d]\n", asm_opt->mid_cnt);
fprintf(stderr, " -1 FILE hap1/paternal k-mer dump generated by \"yak count\" []\n");
fprintf(stderr, " -2 FILE hap2/maternal k-mer dump generated by \"yak count\" []\n");
fprintf(stderr, " -3 FILE list of hap1/paternal read names []\n");
fprintf(stderr, " -4 FILE list of hap2/maternal read names []\n");
fprintf(stderr, " -c INT lower bound of the binned k-mer's frequency [%d]\n", asm_opt->min_cnt);
fprintf(stderr, " -d INT upper bound of the binned k-mer's frequency [%d]\n", asm_opt->mid_cnt);
fprintf(stderr, " Purge-dups:\n");
fprintf(stderr, " -l INT level of purge-dup. In default, [%d] for non-trio; [%d] for trio (see hifiasm.1 for details)\n",
fprintf(stderr, " -l INT level of purge-dup. In default, [%d] for non-trio; [%d] for trio (see hifiasm.1 for details)\n",
asm_opt->purge_level_primary, asm_opt->purge_level_trio);
fprintf(stderr, " -s FLOAT similarity threshold for duplicate haplotigs [%g]\n",
fprintf(stderr, " -s FLOAT similarity threshold for duplicate haplotigs [%g]\n",
asm_opt->purge_simi_rate);
fprintf(stderr, " -O INT min number of overlapped reads for duplicate haplotigs [%d]\n",
fprintf(stderr, " -O INT min number of overlapped reads for duplicate haplotigs [%d]\n",
asm_opt->purge_overlap_len);
fprintf(stderr, " --purge-cov INT coverage upper bound of Purge-dups, which is inferred automatically in default.\n");
fprintf(stderr, "Example: ./hifiasm -o NA12878.asm -t 32 NA12878.fq.gz\n");
@@ -117,8 +123,8 @@ void init_opt(hifiasm_opt_t* asm_opt)
asm_opt->purge_level_trio = 0;
asm_opt->purge_simi_rate = 0.75;
asm_opt->purge_overlap_len = 1;
asm_opt->recover_atg_cov_min = -1;
asm_opt->recover_atg_cov_max = -1;
asm_opt->recover_atg_cov_min = -1024;
asm_opt->recover_atg_cov_max = -1024;
asm_opt->hom_global_coverage = -1;
}
@@ -303,6 +309,19 @@ int check_option(hifiasm_opt_t* asm_opt)
return 0;
}
if(asm_opt->hom_global_coverage < 0 && asm_opt->hom_global_coverage != -1)
{
fprintf(stderr, "[ERROR] purge duplication coverage threshold should be >= 0 (--purge-cov)\n");
return 0;
}
if(asm_opt->recover_atg_cov_min != asm_opt->recover_atg_cov_max &&
(asm_opt->recover_atg_cov_min == -1024 || asm_opt->recover_atg_cov_max == -1024))
{
fprintf(stderr, "[ERROR] primary contig coverage range should be set correctly (--primary-range)\n");
return 0;
}
if(asm_opt->fn_bin_yak[0] != NULL && check_file(asm_opt->fn_bin_yak[0], "YAK1") == 0) return 0;
@@ -411,6 +430,13 @@ int CommandLine_process(int argc, char *argv[], hifiasm_opt_t* asm_opt)
else if (c == 306) asm_opt->max_ov_diff_final = atof(opt.arg);
else if (c == 307) asm_opt->extract_list = opt.arg;
else if (c == 308) asm_opt->extract_iter = atoi(opt.arg);
else if (c == 309) asm_opt->hom_global_coverage = atoi(opt.arg);
else if (c == 310)
{
char* s = NULL;
asm_opt->recover_atg_cov_min = strtol(opt.arg, &s, 10);
if (*s == ',') asm_opt->recover_atg_cov_max = strtol(s + 1, &s, 10);
}
else if (c == 'l')
{ ///0: disable purge_dup; 1: purge containment; 2: purge overlap
asm_opt->purge_level_primary = asm_opt->purge_level_trio = atoi(opt.arg);
@@ -429,6 +455,7 @@ int CommandLine_process(int argc, char *argv[], hifiasm_opt_t* asm_opt)
}
}
if (argc == opt.ind)
{
Print_H(asm_opt);