#include "CommandLines.h" #include #include #include #include "ketopt.h" #include #define VERSION "0.1.0" #define DEFAULT_OUTPUT "hifiasm.asm" hifiasm_opt_t asm_opt; double Get_T(void) { struct timeval t; gettimeofday(&t, NULL); return t.tv_sec+t.tv_usec/1000000.0; } void Print_H(hifiasm_opt_t* asm_opt) { fprintf(stderr, "Usage: hifiasm [options] <...>\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " -o FILE prefix of output files [%s]\n", asm_opt->output_file_name); fprintf(stderr, " -t INT number of threads [%d]\n", asm_opt->thread_num); fprintf(stderr, " -r INT round of correction [%d]\n", asm_opt->number_of_round); fprintf(stderr, " -a INT round of assembly cleaning [%d]\n", asm_opt->clean_round); fprintf(stderr, " -k INT k-mer length [%d] (must be < 64)\n", asm_opt->k_mer_length); ///fprintf(stderr, " -w write all overlaps to disk, can accelerate assembly next time [%d]\n", asm_opt->write_index_to_disk); ///fprintf(stderr, " -l load all overlaps from disk, can avoid overlap calculation [%d]\n", asm_opt->load_index_from_disk); fprintf(stderr, " -i ignore saved overlaps in *.ovlp*.bin files\n"); fprintf(stderr, " -z INT length of adapters that should be removed [%d]\n", asm_opt->adapterLen); fprintf(stderr, " -p INT size of popped bubbles [%lld]\n", asm_opt->pop_bubble_size); fprintf(stderr, " -x FLOAT max overlap drop ratio [%.2g]\n", asm_opt->max_drop_rate); fprintf(stderr, " -y FLOAT min overlap drop ratio [%.2g]\n", asm_opt->min_drop_rate); fprintf(stderr, " -v show version number\n"); fprintf(stderr, " -h show help information\n"); fprintf(stderr, "Example: ./hifiasm -o NA12878.asm -t 32 NA12878.fq.gz\n"); } void init_opt(hifiasm_opt_t* asm_opt) { asm_opt->num_reads = 0; asm_opt->read_file_names = NULL; asm_opt->output_file_name = (char*)(DEFAULT_OUTPUT); asm_opt->required_read_name = NULL; asm_opt->thread_num = 1; asm_opt->k_mer_length = 40; asm_opt->k_mer_min_freq = 3; asm_opt->k_mer_max_freq = 66; asm_opt->load_index_from_disk = 1; asm_opt->write_index_to_disk = 1; asm_opt->number_of_round = 2; asm_opt->adapterLen = 0; asm_opt->clean_round = 4; asm_opt->complete_threads = 0; asm_opt->pop_bubble_size = 100000; asm_opt->min_drop_rate = 0.2; asm_opt->max_drop_rate = 0.8; } void destory_opt(hifiasm_opt_t* asm_opt) { if(asm_opt->read_file_names != NULL) { free(asm_opt->read_file_names); } } void clear_opt(hifiasm_opt_t* asm_opt, int last_round) { asm_opt->complete_threads = 0; asm_opt->num_bases = 0; asm_opt->num_corrected_bases = 0; asm_opt->num_recorrected_bases = 0; asm_opt->roundID = asm_opt->number_of_round - last_round; } int check_option(hifiasm_opt_t* asm_opt) { if(asm_opt->read_file_names == NULL || asm_opt->num_reads == 0) { fprintf(stderr, "[ERROR] missing input: please specify a read file\n"); return 0; } if(asm_opt->output_file_name == NULL) { fprintf(stderr, "[ERROR] missing output: please specify the output name\n"); return 0; } if(asm_opt->thread_num < 1) { fprintf(stderr, "[ERROR] the number of threads must be > 0\n"); return 0; } if(asm_opt->number_of_round < 1) { fprintf(stderr, "[ERROR] the number of rounds for correction must be > 0\n"); return 0; } if(asm_opt->clean_round < 1) { fprintf(stderr, "[ERROR] the number of rounds for assembly cleaning must be > 0\n"); return 0; } if(asm_opt->adapterLen < 0) { fprintf(stderr, "[ERROR] the length of removed adapters must be >= 0\n"); return 0; } if(asm_opt->k_mer_length >= 64) { fprintf(stderr, "[ERROR] the length of k_mer must be < 64\n"); return 0; } if(asm_opt->max_drop_rate < 0 || asm_opt->max_drop_rate >= 1 ) { fprintf(stderr, "[ERROR] max overlap drop ratio must be [0.0, 1.0)\n"); return 0; } if(asm_opt->min_drop_rate < 0 || asm_opt->min_drop_rate >= 1) { fprintf(stderr, "[ERROR] min overlap drop ratio must be [0.0, 1.0)\n"); return 0; } if(asm_opt->max_drop_rate <= asm_opt->min_drop_rate) { fprintf(stderr, "[ERROR] min overlap drop ratio must be less than max overlap drop ratio\n"); return 0; } if(asm_opt->pop_bubble_size < 0) { fprintf(stderr, "[ERROR] the size of popped bubbles must be >= 0\n"); return 0; } // fprintf(stderr, "input file num: %d\n", asm_opt->num_reads); // fprintf(stderr, "output file: %s\n", asm_opt->output_file_name); // fprintf(stderr, "number of threads: %d\n", asm_opt->thread_num); // fprintf(stderr, "number of rounds for correction: %d\n", asm_opt->number_of_round); // fprintf(stderr, "number of rounds for assembly cleaning: %d\n", asm_opt->clean_round); // fprintf(stderr, "length of removed adapters: %d\n", asm_opt->adapterLen); // fprintf(stderr, "length of k_mer: %d\n", asm_opt->k_mer_length); // fprintf(stderr, "min overlap drop ratio: %.2g\n", asm_opt->min_drop_rate); // fprintf(stderr, "max overlap drop ratio: %.2g\n", asm_opt->max_drop_rate); // fprintf(stderr, "size of popped bubbles: %lld\n", asm_opt->pop_bubble_size); return 1; } void get_queries(int argc, char *argv[], ketopt_t* opt, hifiasm_opt_t* asm_opt) { if(opt->ind == argc) { return; } asm_opt->num_reads = argc - opt->ind; asm_opt->read_file_names = (char**)malloc(sizeof(char*)*asm_opt->num_reads); long long i; gzFile dfp; for (i = 0; i < asm_opt->num_reads; i++) { asm_opt->read_file_names[i] = argv[i + opt->ind]; dfp = gzopen(asm_opt->read_file_names[i], "r"); if (dfp == 0) { fprintf(stderr, "[ERROR] Cannot find the input read file: %s\n", asm_opt->read_file_names[i]); exit(0); } gzclose(dfp); } } int CommandLine_process(int argc, char *argv[], hifiasm_opt_t* asm_opt) { ketopt_t opt = KETOPT_INIT; int c; while ((c = ketopt(&opt, argc, argv, 1, "hvt:o:k:lwm:n:r:a:b:z:x:y:p:i", 0)) >= 0) { if (c == 'h') { Print_H(asm_opt); return 0; } else if (c == 'v') { fprintf(stderr, "[Version] %s\n", VERSION); return 0; } else if (c == 't') asm_opt->thread_num = atoi(opt.arg); else if (c == 'o') asm_opt->output_file_name = opt.arg; else if (c == 'n') asm_opt->k_mer_min_freq = atoi(opt.arg); else if (c == 'm') asm_opt->k_mer_max_freq = atoi(opt.arg); else if (c == 'r') asm_opt->number_of_round = atoi(opt.arg); else if (c == 'k') asm_opt->k_mer_length = atoi(opt.arg); else if (c == 'i') asm_opt->load_index_from_disk = 0; else if (c == 'l') asm_opt->load_index_from_disk = 1; else if (c == 'w') asm_opt->write_index_to_disk = 1; else if (c == 'a') asm_opt->clean_round = atoi(opt.arg); else if (c == 'z') asm_opt->adapterLen = atoi(opt.arg); else if (c == 'b') asm_opt->required_read_name = opt.arg; else if (c == 'x') asm_opt->max_drop_rate = atof(opt.arg); else if (c == 'y') asm_opt->min_drop_rate = atof(opt.arg); else if (c == 'p') asm_opt->pop_bubble_size = atoll(opt.arg); else if (c == ':') { fprintf(stderr, "[ERROR] missing option argument in \"%s\"\n", argv[opt.i - 1]); return 0; } else if (c == '?') { fprintf(stderr, "[ERROR] unknown option in \"%s\"\n", argv[opt.i - 1]); return 0; } } if (argc == 1) { Print_H(asm_opt); return 0; } get_queries(argc, argv, &opt, asm_opt); return check_option(asm_opt); }