#include "POA.h" #include #define INIT_EDGE_SIZE 50 #define INCREASE_EDGE_SIZE 5 #define INIT_NODE_SIZE 16000 void check_addUnmatchedSeqToGraph(Graph* g, char* g_read_seq, long long g_read_length, long long startID, long long endID) { long long reverse_startID, reverse_endID; reverse_startID = startID; reverse_endID = endID; long long i = 0; if (endID - startID + 1 != g_read_length) { fprintf(stderr, "ERROR Length...\n"); fprintf(stderr, "startID: %lld, endID: %lld\n", startID, endID); fprintf(stderr, "g_read_length: %lld\n", g_read_length); } if (g_read_length == 0) { return; } while (1) { if(g->g_nodes.list[startID].base != g_read_seq[i]) { fprintf(stderr, "i: %llu, ERROR Node Base...\n", i); } if(g->g_nodes.list[startID].outcome_edges.length == 0) { break; } startID = g->g_nodes.list[startID].outcome_edges.list[0].out_node; i++; } if (startID != endID) { fprintf(stderr, "ERROR End Node Base\n"); } i = g_read_length - 1; while (1) { if(g->g_nodes.list[reverse_endID].base != g_read_seq[i]) { fprintf(stderr, "i: %llu, g_read_length: %llu, ERROR Node Base...\n", i, g_read_length); } if(g->g_nodes.list[reverse_endID].income_edges.length == 0) { break; } reverse_endID = g->g_nodes.list[reverse_endID].income_edges.list[0].in_node; i--; } if (reverse_startID != reverse_endID) { fprintf(stderr, "ERROR Start Node Base\n"); } } void init_Edge_alloc(Edge_alloc* list) { if (list->list == NULL) { list->size = INIT_EDGE_SIZE; list->length = 0; list->list = (Edge*)malloc(sizeof(Edge)*list->size); } else { list->length = 0; } } void clear_Edge_alloc(Edge_alloc* list) { list->length = 0; } void destory_Edge_alloc(Edge_alloc* list) { free(list->list); } void append_Edge_alloc(Edge_alloc* list, uint64_t in_node, uint64_t out_node, uint64_t weight) { if (list->length + 1 > list->size) { list->size = list->size + INCREASE_EDGE_SIZE; list->list = (Edge*)realloc(list->list, sizeof(Edge)*list->size); } list->list[list->length].in_node = in_node; list->list[list->length].out_node = out_node; list->list[list->length].weight = weight; list->length++; } void init_Node_alloc(Node_alloc* list) { list->size = INIT_NODE_SIZE; list->length = 0; list->list = (Node*)malloc(sizeof(Node)*list->size); list->sort.size = 0; list->sort.list = NULL; list->sort.visit = NULL; list->sort.iterative_buffer = NULL; list->sort.iterative_buffer_visit = NULL; long long i; for (i = 0; i < list->size; i++) { list->list[i].income_edges.list=NULL; list->list[i].outcome_edges.list=NULL; list->list[i].alignedTo_Nodes.list=NULL; } } void destory_Node_alloc(Node_alloc* list) { uint64_t i =0; for (i = 0; i < list->length; i++) { destory_Edge_alloc(&list->list[i].income_edges); destory_Edge_alloc(&list->list[i].outcome_edges); destory_Edge_alloc(&list->list[i].alignedTo_Nodes); } free(list->list); free(list->sort.list); free(list->sort.visit); free(list->sort.iterative_buffer); free(list->sort.iterative_buffer_visit); ///free(list->topo_order); } void clear_Node_alloc(Node_alloc* list) { uint64_t i =0; for (i = 0; i < list->length; i++) { clear_Edge_alloc(&list->list[i].income_edges); clear_Edge_alloc(&list->list[i].outcome_edges); clear_Edge_alloc(&list->list[i].alignedTo_Nodes); } list->length = 0; } uint64_t append_Node_alloc(Node_alloc* list, char base) { if (list->length + 1 > list->size) { long long i = list->size; ///list->topo_order这里用不到,所以不用先分配空间 ///但是还是一起分配了吧,免得麻烦 list->size = list->size * 2; list->list = (Node*)realloc(list->list, sizeof(Node)*list->size); ///list->topo_order = (uint64_t*)realloc(list->topo_order, sizeof(uint64_t)*list->size); for (; i < list->size; i++) { list->list[i].income_edges.list=NULL; list->list[i].outcome_edges.list=NULL; list->list[i].alignedTo_Nodes.list=NULL; } } list->list[list->length].ID = list->length; list->list[list->length].base = base; init_Edge_alloc(&list->list[list->length].income_edges); init_Edge_alloc(&list->list[list->length].outcome_edges); init_Edge_alloc(&list->list[list->length].alignedTo_Nodes); list->length++; return list->length - 1; } void init_Graph(Graph* g) { init_Node_alloc(&g->g_nodes); g->g_n_edges = 0; g->g_n_nodes = 0; g->g_next_nodeID = 0; g->s_end_nodeID = 0; g->s_start_nodeID = 0; g->seq = NULL; g->seqID = (uint64_t)-1; } void destory_Graph(Graph* g) { destory_Node_alloc(&g->g_nodes); } void clear_Graph(Graph* g) { clear_Node_alloc(&g->g_nodes); g->g_n_edges = 0; g->g_n_nodes = 0; g->g_next_nodeID = 0; g->s_end_nodeID = 0; g->s_start_nodeID = 0; g->seq = NULL; g->seqID = (uint64_t)-1; } uint64_t inline add_Node_Graph(Graph* g, char base) { return append_Node_alloc(&g->g_nodes, base); } void inline add_Edge_Graph(Graph* g, uint64_t start, uint64_t end, uint64_t weight) { if (start >= g->g_nodes.length || end >= g->g_nodes.length) { fprintf(stderr, "Not existing nodes ..."); exit(0); } ///对起始节点加出边 append_Edge_alloc(&g->g_nodes.list[start].outcome_edges, start, end, weight); ///对结束节点加入边 append_Edge_alloc(&g->g_nodes.list[end].income_edges, start, end, weight); } void addUnmatchedSeqToGraph(Graph* g, char* g_read_seq, long long g_read_length, long long* startID, long long* endID) { long long firstID, lastID, nodeID, i; firstID = -1; lastID = -1; if(g_read_length == 0) return; for (i = 0; i < g_read_length; i++) { nodeID = add_Node_Graph(g, g_read_seq[i]); ////fprintf(stderr, "nodeID: %llu\n", nodeID); if (firstID == -1) { firstID = nodeID; } if (lastID != -1) { add_Edge_Graph(g, lastID, nodeID, 1); } lastID = nodeID; } *startID = firstID; *endID = lastID; } void Perform_POA(Graph* g, overlap_region_alloc* overlap_list, All_reads* R_INF, UC_Read* g_read) { long long startNodeID, endNodeID; ///第一条序列是read本身,g_read里存的是反向互补,所以首先要恢复回正向 ///这一步可以优化掉 reverse_complement(g_read->seq, g_read->length); addUnmatchedSeqToGraph(g, g_read->seq, g_read->length, &startNodeID, &endNodeID); /** if (startNodeID!=0||endNodeID!=g_read->length-1) { fprintf(stderr, "Error startNodeID or endNodeID ...\n"); } check_addUnmatchedSeqToGraph(g, g_read->seq, g_read->length, startNodeID, endNodeID); **/ get_Topo_Sort_Order(&g->g_nodes, 0); uint64_t i = 0; for ( i = 0; i < overlap_list->length; i++) { /** if(overlap_list->list[i].x_id == overlap_list->list[i].y_id) { fprintf(stderr, "Error x_id or y_id ...\n"); } **/ if (overlap_list->list[i].y_pos_strand) { recover_UC_Read_RC(g_read, R_INF, overlap_list->list[i].y_id); } else { recover_UC_Read(g_read, R_INF, overlap_list->list[i].y_id); } } } void topologicalSortDFS(Node_alloc* list, uint64_t nodeID) { list->sort.visit[nodeID] = 1; long long i; uint64_t out_nodeID; for (i = 0; i < list->list[nodeID].outcome_edges.length; i++) { out_nodeID = list->list[nodeID].outcome_edges.list[i].out_node; if (list->sort.visit[out_nodeID] == 0) { topologicalSortDFS(list, out_nodeID); } } list->sort.length--; list->sort.list[list->sort.length] = nodeID; } #define INIT_STACK(stack) stack.iterative_i = 0; #define PUSH(stack, nodeID, time) stack.iterative_buffer[stack.iterative_i]=nodeID;\ stack.iterative_buffer_visit[stack.iterative_i++]=time; #define IF_EMPTY(stack) (stack.iterative_i == 0) #define POP(stack, nodeID, time) --stack.iterative_i;nodeID = stack.iterative_buffer[stack.iterative_i];\ time = stack.iterative_buffer_visit[stack.iterative_i]; void topologicalSortDFS_Iterative(Node_alloc* list, uint64_t nodeID) { long long i; uint64_t out_nodeID; int flag; INIT_STACK(list->sort); PUSH(list->sort, nodeID, 0); while (!IF_EMPTY(list->sort)) { POP(list->sort, nodeID, flag); ///flag == 1说明是第二次访问; flag == 0说明是第一次访问 if (flag) { list->sort.length--; list->sort.list[list->sort.length] = nodeID; continue; } list->sort.visit[nodeID] = 1; PUSH(list->sort, nodeID, 1); for (i = 0; i < list->list[nodeID].outcome_edges.length; i++) { out_nodeID = list->list[nodeID].outcome_edges.list[i].out_node; if (list->sort.visit[out_nodeID] == 0) { PUSH(list->sort, out_nodeID, 0); } } } } uint64_t* get_Topo_Sort_Order(Node_alloc* list, int need_sort) { long long i = 0; list->sort.length = list->length; if (list->length > list->sort.size) { list->sort.size = list->length; list->sort.list = (uint64_t*)realloc(list->sort.list, sizeof(uint64_t)*list->sort.size); list->sort.visit = (uint8_t*)realloc(list->sort.visit, sizeof(uint8_t)*list->sort.size); list->sort.iterative_buffer = (uint64_t*)realloc(list->sort.iterative_buffer, sizeof(uint64_t)*list->sort.size); list->sort.iterative_buffer_visit = (uint8_t*)realloc(list->sort.iterative_buffer_visit, sizeof(uint8_t)*list->sort.size); } if (!need_sort) { ///可以循环展开, 作用微乎其微 for (i = 0; i < list->length; i++) { list->sort.list[i] = i; } } else { memset(list->sort.visit, 0 , list->length); for (i = 0; i < list->length; i++) { if(list->sort.visit[i] == 0) { ///topologicalSortDFS(list, i); topologicalSortDFS_Iterative(list, i); } } /** for (i = 0; i < list->length; i++) { if (list->sort.list[i] != i) { fprintf(stderr, "ERROR Sort ....\n"); } } **/ } return list->sort.list; }