#ifndef __HASHTABLE__ #define __HASHTABLE__ #include "htab.h" #define PREFIX_BITS 16 #define MAX_SUFFIX_BITS 64 #define MODE_VALUE 101 ///#define WINDOW 350 ///#define THRESHOLD 14 #define WINDOW 375 //#define WINDOW_BOUNDARY 150 #define WINDOW_BOUNDARY 375 ///for one side, the first or last WINDOW_UNCORRECT_SINGLE_SIDE_BOUNDARY bases should not be corrected #define WINDOW_UNCORRECT_SINGLE_SIDE_BOUNDARY 25 #define THRESHOLD 15 #define THRESHOLD_RATE 0.04 #define TAIL_LENGTH int(1/THRESHOLD_RATE) ///#define OVERLAP_THRESHOLD 0.9 #define OVERLAP_THRESHOLD_FILTER 0.9 #define WINDOW_MAX_SIZE WINDOW + TAIL_LENGTH + 3 #define THRESHOLD_MAX_SIZE 31 #define FINAL_OVERLAP_ERROR_RATE 0.03 #define GROUP_SIZE 4 ///the max cigar likes 10M10D10M10D10M ///#define CIGAR_MAX_LENGTH THRESHOLD*2+2 #define CIGAR_MAX_LENGTH 31*2+4 typedef struct { uint32_t offset; uint32_t readID:31, rev:1; } k_mer_pos; typedef struct { k_mer_pos* list; uint64_t length; uint64_t size; uint8_t direction; uint64_t end_pos; } k_mer_pos_list; typedef struct { int C_L[CIGAR_MAX_LENGTH]; char C_C[CIGAR_MAX_LENGTH]; int length; } CIGAR; typedef struct { ///the begining and end of a window, instead of the whole overlap uint64_t x_start; uint64_t x_end; int y_end; int y_start; int extra_begin; int extra_end; int error_threshold; int error; CIGAR cigar; } window_list; typedef struct { window_list* buffer; int32_t length; int32_t size; } window_list_alloc; typedef struct { uint64_t* buffer; uint32_t length; uint32_t size; } Fake_Cigar; typedef struct { uint32_t x_id; ///the begining and end of the whole overlap uint32_t x_pos_s; uint32_t x_pos_e; uint32_t x_pos_strand; uint32_t y_id; uint32_t y_pos_s; uint32_t y_pos_e; uint32_t y_pos_strand; uint32_t overlapLen; uint32_t shared_seed; uint32_t align_length; uint8_t is_match; uint8_t without_large_indel; int8_t strong; uint32_t non_homopolymer_errors; window_list* w_list; uint32_t w_list_size; uint32_t w_list_length; Fake_Cigar f_cigar; window_list_alloc boundary_cigars; } overlap_region; typedef struct { overlap_region* list; uint64_t size; uint64_t length; int64_t mapped_overlaps_length; } overlap_region_alloc; typedef struct { uint32_t readID:31, strand:1; uint32_t offset, self_offset; } k_mer_hit; typedef struct { int32_t *score; int64_t *pre; int32_t *indels; int32_t *self_length; int64_t *tmp; // MUST BE 64-bit integer int64_t length; int64_t size; } Chain_Data; typedef struct { k_mer_hit* list; long long length; long long size; Chain_Data chainDP; } Candidates_list; void init_Candidates_list(Candidates_list* l); void clear_Candidates_list(Candidates_list* l); void destory_Candidates_list(Candidates_list* l); void init_overlap_region_alloc(overlap_region_alloc* list); void clear_overlap_region_alloc(overlap_region_alloc* list); void destory_overlap_region_alloc(overlap_region_alloc* list); void append_window_list(overlap_region* region, uint64_t x_start, uint64_t x_end, int y_start, int y_end, int error, int extra_begin, int extra_end, int error_threshold); void overlap_region_sort_y_id(overlap_region *a, long long n); void calculate_overlap_region_by_chaining(Candidates_list* candidates, overlap_region_alloc* overlap_list, uint64_t readID, uint64_t readLength, All_reads* R_INF, double band_width_threshold, int add_beg_end); void init_fake_cigar(Fake_Cigar* x); void destory_fake_cigar(Fake_Cigar* x); void clear_fake_cigar(Fake_Cigar* x); void add_fake_cigar(Fake_Cigar* x, uint32_t gap_site, int32_t gap_shift); void resize_fake_cigar(Fake_Cigar* x, uint64_t size); int get_fake_gap_pos(Fake_Cigar* x, int index); int get_fake_gap_shift(Fake_Cigar* x, int index); static inline long long y_start_offset(long long x_start, Fake_Cigar* o) { if(x_start == get_fake_gap_pos(o, o->length - 1)) { return get_fake_gap_shift(o, o->length - 1); } long long i; for (i = 0; i < (long long)o->length; i++) { if(x_start < get_fake_gap_pos(o, i)) { break; } } if(i == 0 || i == (long long)o->length) { fprintf(stderr, "ERROR at %s:%d\n", __FILE__, __LINE__); exit(0); } ///note here return i - 1 return get_fake_gap_shift(o, i - 1); } void resize_Chain_Data(Chain_Data* x, long long size); void init_window_list_alloc(window_list_alloc* x); void clear_window_list_alloc(window_list_alloc* x); void destory_window_list_alloc(window_list_alloc* x); void resize_window_list_alloc(window_list_alloc* x, long long size); void chain_DP(k_mer_hit* a, long long a_n, Chain_Data* dp, overlap_region* result, double band_width_threshold, int max_skip, int x_readLen, int y_readLen); #endif