#include #include #include #include "Hash_Table.h" void init_k_mer_pos_list_alloc(k_mer_pos_list_alloc* list) { list->size = 1000; list->length = 0; list->list = (k_mer_pos_list*)malloc(sizeof(k_mer_pos_list)*list->size); } void destory_k_mer_pos_list_alloc(k_mer_pos_list_alloc* list) { free(list->list); } void clear_k_mer_pos_list_alloc(k_mer_pos_list_alloc* list) { list->length = 0; } void append_k_mer_pos_list_alloc(k_mer_pos_list_alloc* list, k_mer_pos* n_list, uint64_t n_length) { list->length++; if (list->length > list->size) { list->size = list->size * 2; list->list = (k_mer_pos_list*)realloc(list->list, sizeof(k_mer_pos_list)*list->size); } list->list[list->length - 1].list = n_list; list->list[list->length - 1].length = n_length; } void init_Count_Table(Count_Table** table) { *table = kh_init(COUNT64); } void init_Pos_Table(Pos_Table** table) { *table = kh_init(POS64); } void init_Total_Count_Table(int k, Total_Count_Table* TCB) { if(k>64) { fprintf(stdout, "k-mer is too long. The length of k-mer must <= 64."); fflush(stdout); exit(0); } int total_bits = k * 2; TCB->prefix_bits = PREFIX_BITS; TCB->suffix_bits = total_bits - TCB->prefix_bits; if (TCB->suffix_bits > MAX_SUFFIX_BITS) { TCB->suffix_bits = MAX_SUFFIX_BITS; TCB->prefix_bits = total_bits - TCB->suffix_bits; } ///TCB->suffix_mode = (1ULL<suffix_bits) - 1; ///这个右移是安全的,因为TCB->suffix_bits不可能是0 TCB->suffix_mode = ALL >> (64 - TCB->suffix_bits); ///number of small hash table TCB->size = (1ULL<prefix_bits); TCB->sub_h = (Count_Table**)malloc(sizeof(Count_Table*)*TCB->size); TCB->sub_h_lock = (Hash_table_spin_lock*)malloc(sizeof(Hash_table_spin_lock)*TCB->size); memset(TCB->sub_h_lock, 0, sizeof(Hash_table_spin_lock)*TCB->size); int i = 0; for (i = 0; i < TCB->size; i++) { init_Count_Table(&(TCB->sub_h[i])); TCB->sub_h_lock[i].lock = 0; } TCB->non_unique_k_mer = 0; } void init_Total_Pos_Table(Total_Pos_Table* TCB, Total_Count_Table* pre_TCB) { TCB->prefix_bits = pre_TCB->prefix_bits; TCB->suffix_bits = pre_TCB->suffix_bits; TCB->suffix_mode = pre_TCB->suffix_mode; TCB->size = pre_TCB->size; TCB->useful_k_mer = 0; TCB->total_occ = 0; TCB->k_mer_index = NULL; TCB->sub_h_lock = (Hash_table_spin_lock*)malloc(sizeof(Hash_table_spin_lock)*TCB->size); memset(TCB->sub_h_lock, 0, sizeof(Hash_table_spin_lock)*TCB->size); TCB->sub_h = (Pos_Table**)malloc(sizeof(Pos_Table*)*TCB->size); TCB->pos = NULL; int i = 0; for (i = 0; i < TCB->size; i++) { init_Pos_Table(&(TCB->sub_h[i])); TCB->sub_h_lock[i].lock = 0; } } void destory_Total_Count_Table(Total_Count_Table* TCB) { int i; for (i = 0; i < TCB->size; i++) { kh_destroy(COUNT64, TCB->sub_h[i]); } free(TCB->sub_h); free(TCB->sub_h_lock); } void destory_Total_Pos_Table(Total_Pos_Table* TCB) { free(TCB->k_mer_index); free(TCB->sub_h_lock); free(TCB->pos); int i; for (i = 0; i < TCB->size; i++) { kh_destroy(POS64, TCB->sub_h[i]); } free(TCB->sub_h); } void write_Total_Pos_Table(Total_Pos_Table* TCB, char* read_file_name) { fprintf(stdout, "Writing index to disk ...... \n"); char* index_name = (char*)malloc(strlen(read_file_name)+5); sprintf(index_name, "%s.idx", read_file_name); FILE* fp = fopen(index_name, "w"); fwrite(&TCB->prefix_bits, sizeof(TCB->prefix_bits), 1, fp); fwrite(&TCB->suffix_bits, sizeof(TCB->suffix_bits), 1, fp); fwrite(&TCB->suffix_mode, sizeof(TCB->suffix_mode), 1, fp); fwrite(&TCB->size, sizeof(TCB->size), 1, fp); fwrite(&TCB->useful_k_mer, sizeof(TCB->useful_k_mer), 1, fp); fwrite(&TCB->total_occ, sizeof(TCB->total_occ), 1, fp); fwrite(TCB->k_mer_index, sizeof(uint64_t), TCB->useful_k_mer+1, fp); fwrite(TCB->pos, sizeof(k_mer_pos), TCB->total_occ, fp); int i; for (i = 0; i < TCB->size; i++) { kh_write(POS64, TCB->sub_h[i], fp); } free(index_name); fclose(fp); fprintf(stdout, "Index has been written.\n"); } int load_Total_Pos_Table(Total_Pos_Table* TCB, char* read_file_name) { fprintf(stdout, "Loading index to disk ...... \n"); char* index_name = (char*)malloc(strlen(read_file_name)+5); sprintf(index_name, "%s.idx", read_file_name); FILE* fp = fopen(index_name, "r"); if (!fp) { return 0; } fread(&TCB->prefix_bits, sizeof(TCB->prefix_bits), 1, fp); fread(&TCB->suffix_bits, sizeof(TCB->suffix_bits), 1, fp); fread(&TCB->suffix_mode, sizeof(TCB->suffix_mode), 1, fp); fread(&TCB->size, sizeof(TCB->size), 1, fp); fread(&TCB->useful_k_mer, sizeof(TCB->useful_k_mer), 1, fp); fread(&TCB->total_occ, sizeof(TCB->total_occ), 1, fp); if (TCB->useful_k_mer+1) { TCB->k_mer_index = (uint64_t*)malloc(sizeof(uint64_t)*(TCB->useful_k_mer+1)); fread(TCB->k_mer_index, sizeof(uint64_t), TCB->useful_k_mer+1, fp); } else { TCB->k_mer_index = NULL; } if (TCB->total_occ) { TCB->pos = (k_mer_pos*)malloc(sizeof(k_mer_pos)*TCB->total_occ); fread(TCB->pos, sizeof(k_mer_pos), TCB->total_occ, fp); } else { TCB->pos = NULL; } TCB->sub_h_lock = (Hash_table_spin_lock*)malloc(sizeof(Hash_table_spin_lock)*TCB->size); memset(TCB->sub_h_lock, 0, sizeof(Hash_table_spin_lock)*TCB->size); TCB->sub_h = (Pos_Table**)malloc(sizeof(Pos_Table*)*TCB->size); int i; for (i = 0; i < TCB->size; i++) { init_Pos_Table(&(TCB->sub_h[i])); TCB->sub_h_lock[i].lock = 0; kh_load(POS64, TCB->sub_h[i], fp); } free(index_name); fclose(fp); fprintf(stdout, "Index has been loaded.\n"); return 1; } void Traverse_Counting_Table(Total_Count_Table* TCB, Total_Pos_Table* PCB, int k_mer_min_freq, int k_mer_max_freq) { int i; Count_Table* h; khint_t k; uint64_t sub_key; uint64_t sub_ID; PCB->useful_k_mer = 0; PCB->total_occ = 0; ///init_Total_Pos_Table(PCB, TCB); khint_t t; ///这就是个迭代器 int absent; for (i = 0; i < TCB->size; i++) { h = TCB->sub_h[i]; for (k = kh_begin(h); k != kh_end(h); ++k) { if (kh_exist(h, k)) // test if a bucket contains data { ///只有符合频率范围要求的k-mer,才会被加入到pos table中 if (kh_value(h, k)>=k_mer_min_freq && kh_value(h, k)<=k_mer_max_freq) { sub_ID = i; sub_key = kh_key(h, k); t = kh_put(POS64, PCB->sub_h[sub_ID], sub_key, &absent); if (absent) { ///kh_value(PCB->sub_h[sub_ID], t) = useful_k_mer + total_occ; kh_value(PCB->sub_h[sub_ID], t) = PCB->useful_k_mer; } else ///哈希表中已有的元素 { ///kh_value(PCB->sub_h[sub_ID], t)++; fprintf(stderr, "ERROR\n"); } PCB->useful_k_mer++; PCB->total_occ = PCB->total_occ + kh_value(h, k); } } } } fprintf(stdout, "useful_k_mer: %lld\n",PCB->useful_k_mer); fprintf(stdout, "total_occ: %lld\n",PCB->total_occ); PCB->k_mer_index = (uint64_t*)malloc(sizeof(uint64_t)*(PCB->useful_k_mer+1)); PCB->k_mer_index[0] = 0; PCB->total_occ = 0; PCB->useful_k_mer = 0; for (i = 0; i < TCB->size; i++) { h = TCB->sub_h[i]; for (k = kh_begin(h); k != kh_end(h); ++k) { if (kh_exist(h, k)) // test if a bucket contains data { if (kh_value(h, k)>=k_mer_min_freq && kh_value(h, k)<=k_mer_max_freq) { PCB->useful_k_mer++; PCB->total_occ = PCB->total_occ + kh_value(h, k); PCB->k_mer_index[PCB->useful_k_mer] = PCB->total_occ; } } } } PCB->pos = (k_mer_pos*)malloc(sizeof(k_mer_pos)*PCB->total_occ); memset(PCB->pos, 0, sizeof(k_mer_pos)*PCB->total_occ); } int cmp_k_mer_pos(const void * a, const void * b) { if ((*(k_mer_pos*)a).readID != (*(k_mer_pos*)b).readID) { return (*(k_mer_pos*)a).readID > (*(k_mer_pos*)b).readID ? 1 : -1; } else { if ((*(k_mer_pos*)a).offset != (*(k_mer_pos*)b).offset) { return (*(k_mer_pos*)a).offset > (*(k_mer_pos*)b).offset ? 1 : -1; } else { return 0; } } } void init_Candidates_list(Candidates_list* l) { l->length = 0; l->size = 0; l->list = NULL; l->tmp = NULL; l->foward_pos = 0; l->rc_pos = 0; } void clear_Candidates_list(Candidates_list* l) { l->length = 0; l->foward_pos = 0; l->rc_pos = 0; } void destory_Candidates_list(Candidates_list* l) { free(l->list); free(l->tmp); } ///1是x小,2是y小,0是相等 inline int cmp_pos(k_mer_hit* x, k_mer_pos* y, uint64_t y_pos, uint8_t strand) { if (x->strand < strand) { return 1; } else if (x->strand > strand) { return 2; } else { if (x->readID < y->readID) { return 1; } else if (x->readID > y->readID) { return 2; } else { if (x->offset < y->offset) { return 1; } else if (x->offset > y->offset) { return 2; } else { if (x->self_offset < y_pos) { return 1; } else if (x->self_offset > y_pos) { return 2; } else { return 0; } } } } } void debug_merge_Candidates_list(Candidates_list* l, k_mer_pos* n_list, uint64_t n_lengh, uint64_t end_pos, uint64_t i, uint64_t i_1, uint64_t i_2, uint64_t len1, uint64_t len2, uint64_t strand) { if (i != l->length) { fprintf(stderr, "ERROR\n"); fprintf(stderr, "i: %llu, l->length: %llu\n", i , l->length); fprintf(stderr, "i_1: %llu, len1: %llu\n", i_1 , len1); fprintf(stderr, "i_2: %llu, len2: %llu\n", i_2 , len2); fprintf(stderr, "strand: %llu\n", strand); } for (i = 1; i < l->length; i++) { if (l->list[i].strand < l->list[i-1].strand) { fprintf(stderr, "ERROR -1\n"); } else if (l->list[i].strand == l->list[i-1].strand) { if (l->list[i].readID < l->list[i-1].readID) { fprintf(stderr, "ERROR 0\n"); } else if (l->list[i].readID == l->list[i-1].readID) { if (l->list[i].offset < l->list[i-1].offset) { fprintf(stderr, "ERROR 1\n"); } else if (l->list[i].offset == l->list[i-1].offset) { if (l->list[i].self_offset < l->list[i-1].self_offset) { fprintf(stderr, "ERROR 2\n"); } } } } } for (i = 0; i < n_lengh; i++) { int j = 0; for (j = 0; j < l->length; j++) { if ( n_list[i].offset == l->list[j].offset && n_list[i].readID == l->list[j].readID && end_pos == l->list[j].self_offset && strand == l->list[j].strand ) { break; } } if (j == l->length) { fprintf(stderr, "ERROR 4\n"); } } for (i = 0; i < l->length - n_lengh; i++) { int j = 0; for (j = 0; j < l->length; j++) { if ( l->tmp[i].offset == l->list[j].offset && l->tmp[i].readID == l->list[j].readID && l->tmp[i].self_offset == l->list[j].self_offset && l->tmp[i].strand == l->list[j].strand ) { break; } } if (j == l->length) { fprintf(stderr, "ERROR 5\n"); } } } void merge_Candidates_list(Candidates_list* l, k_mer_pos* n_list, uint64_t n_lengh, uint64_t end_pos, int strand) { if (n_lengh == 0) ///不加这个就会巨慢 { return; } if(l->length + n_lengh > l->size) { l->size = l->length + n_lengh; l->list = (k_mer_hit*)realloc(l->list, sizeof(k_mer_hit)*l->size); l->tmp = (k_mer_hit*)realloc(l->tmp, sizeof(k_mer_hit)*l->size); } int flag; uint64_t len1, len2, i_1, i_2, i; k_mer_hit *l_1; k_mer_pos *l_2; l_1 = l->list; if (strand == 0) { ///由于标号全是0,则标号为1的位置不受影响(数组后半部分),可以直接复制过去 ///需要从l_1 + l->rc_pos开始,复制l->length-l->rc_pos个元素,到l->tmp + l->rc_pos + n_lengh处 memcpy(l->tmp + l->rc_pos + n_lengh, l_1 + l->rc_pos, sizeof(k_mer_hit)*(l->length-l->rc_pos)); ///l_1只需要从0扫描到l->rc_pos就好了 len1 = l->rc_pos; i_1 = 0; ///此时目标数组没数据, i = 0; ///更新l->rc_pos l->rc_pos = l->rc_pos + n_lengh; } else { ///如果strand==1,则标号为0的位置不受影响(数组前半部分),可以直接复制过去 ///从l_1开始,复制l->rc_pos个元素,到l->tmp memcpy(l->tmp, l_1, sizeof(k_mer_hit)*l->rc_pos); ///l_1只需要从l->rc_pos扫描到末尾就好了 i_1 = l->rc_pos; len1 = l->length; ///此时目标数组已经有了l->rc_pos i = i_1; ///l->rc_pos不用更新 } l_2 = n_list; len2 = n_lengh; i_2 = 0; while (i_1tmp[i].readID = l_1[i_1].readID; l->tmp[i].offset = l_1[i_1].offset; l->tmp[i].self_offset = l_1[i_1].self_offset; l->tmp[i].strand = l_1[i_1].strand; i_1++; i++; } else ///l_2小或者l_1 == l_2 { l->tmp[i].readID = l_2[i_2].readID; l->tmp[i].offset = l_2[i_2].offset; l->tmp[i].self_offset = end_pos; l->tmp[i].strand = strand; i_2++; i++; } } while (i_1tmp + i, l_1 + i_1, sizeof(k_mer_hit)*(len1-i_1)); i = i + len1-i_1; i_1 = len1; } while (i_2tmp[i].readID = l_2[i_2].readID; l->tmp[i].offset = l_2[i_2].offset; l->tmp[i].self_offset = end_pos; l->tmp[i].strand = strand; i_2++; i++; } k_mer_hit* k; k = l->list; l->list = l->tmp; l->tmp = k; l->length = l->length + n_lengh; /** if (strand == 0) { i = l->length; len1 = l->length - n_lengh; } debug_merge_Candidates_list(l, n_list, n_lengh, end_pos, i, i_1, i_2, len1, len2, strand); **/ } /********************************for debug***************************************/ void merge_Candidates_list_version(Candidates_list* l, k_mer_pos* n_list, uint64_t n_lengh, uint64_t end_pos, int strand) { if (n_lengh == 0) ///不加这个就会巨慢 { return; } if(l->length + n_lengh > l->size) { l->size = l->length + n_lengh; l->list = (k_mer_hit*)realloc(l->list, sizeof(k_mer_hit)*l->size); l->tmp = (k_mer_hit*)realloc(l->tmp, sizeof(k_mer_hit)*l->size); } int flag; uint64_t len1, len2, i_1, i_2, i; k_mer_hit *l_1; k_mer_pos *l_2; l_1 = l->list; len1 = l->length; i_1 = 0; l_2 = n_list; len2 = n_lengh; i_2 = 0; i = 0; while (i_1tmp[i].readID = l_1[i_1].readID; l->tmp[i].offset = l_1[i_1].offset; l->tmp[i].self_offset = l_1[i_1].self_offset; l->tmp[i].strand = l_1[i_1].strand; i_1++; i++; } else ///l_2小或者l_1 == l_2 { l->tmp[i].readID = l_2[i_2].readID; l->tmp[i].offset = l_2[i_2].offset; l->tmp[i].self_offset = end_pos; l->tmp[i].strand = strand; i_2++; i++; } } while (i_1tmp[i].readID = l_1[i_1].readID; l->tmp[i].offset = l_1[i_1].offset; l->tmp[i].self_offset = l_1[i_1].self_offset; l->tmp[i].strand = l_1[i_1].strand; i_1++; i++; } while (i_2tmp[i].readID = l_2[i_2].readID; l->tmp[i].offset = l_2[i_2].offset; l->tmp[i].self_offset = end_pos; l->tmp[i].strand = strand; i_2++; i++; } k_mer_hit* k; k = l->list; l->list = l->tmp; l->tmp = k; l->length = l->length + n_lengh; ///debug_merge_Candidates_list(l, n_list, n_lengh, end_pos, i, i_1, i_2, len1, len2, strand); } /********************************for debug***************************************/ void debug_mode(uint64_t d, uint64_t thread_ID, uint64_t thread_num) { fprintf(stderr, "#%llu: Start debug_mode...\n", thread_ID); uint64_t i; for (i = thread_ID; i < 4,294,967,296; i = i + thread_num) { if(i%d != mod_d(0, i, d)) { fprintf(stderr, "ERROR mode, i: %llu\n", i); fprintf(stderr, "i mod d: %llu\n", i%d); fprintf(stderr, "mod_d(0, i, d): %llu\n", mod_d(0, i, d)); } } fprintf(stderr, "#%llu: Finish debug_mode\n", thread_ID); } /********************************for debug***************************************/ void test_COUNT64() { uint64_t sb[7] = {5000000000, 6000000000, 7000000000, 8000000000, 9000000000, 10000000000 ,5000000000}; Count_Table* h; ///构建哈希表 init_Count_Table(&h); int i; khint_t k; ///这就是个迭代器 int absent; for (i = 0; i < 7; i++) { ///将5作为key插入到哈希表COUNT64中 ///absent为0代表哈希表里面已经有这个key了 k = kh_put(COUNT64, h, sb[i], &absent); ///代表插入了一个哈希表中没有的元素 ///则直接插入 if (absent) { kh_value(h, k) = i; } else ///哈希表中已有的元素 { kh_value(h, k) = i; } } for (i = 0; i < 7; i++) { ///查询哈希表,key为k k = kh_get(COUNT64, h, sb[i]); if (k != kh_end(h)) { fprintf(stderr, "value: %d\n", kh_value(h, k)); } else { fprintf(stderr, "not found!\n"); } } kh_destroy(COUNT64, h); }