#include #include "htab.h" #include "ksort.h" #include "Hash_Table.h" typedef struct { // this struct is not strictly necessary; we can use k_mer_pos instead, with modifications uint64_t srt; uint32_t self_off; uint32_t other_off; } anchor1_t; #define an_key1(a) ((a).srt) #define an_key2(a) ((a).self_off) KRADIX_SORT_INIT(ha_an1, anchor1_t, an_key1, 8) KRADIX_SORT_INIT(ha_an2, anchor1_t, an_key2, 4) #define oreg_xs_lt(a, b) (((uint64_t)(a).x_pos_s<<32|(a).x_pos_e) < ((uint64_t)(b).x_pos_s<<32|(b).x_pos_e)) KSORT_INIT(or_xs, overlap_region, oreg_xs_lt) #define oreg_ss_lt(a, b) ((a).shared_seed > (b).shared_seed) // in the decending order KSORT_INIT(or_ss, overlap_region, oreg_ss_lt) typedef struct { int n; const ha_idxpos_t *a; } seed1_t; struct ha_abuf_s { uint64_t n_a, m_a; uint32_t old_mz_m; ha_mz1_v mz; seed1_t *seed; anchor1_t *a; }; ha_abuf_t *ha_abuf_init(void) { return (ha_abuf_t*)calloc(1, sizeof(ha_abuf_t)); } void ha_abuf_destroy(ha_abuf_t *ab) { free(ab->seed); free(ab->a); free(ab->mz.a); free(ab); } uint64_t ha_abuf_mem(const ha_abuf_t *ab) { return ab->m_a * sizeof(anchor1_t) + ab->mz.m * (sizeof(ha_mz1_t) + sizeof(seed1_t)) + sizeof(ha_abuf_t); } void ha_get_new_candidates(ha_abuf_t *ab, int64_t rid, UC_Read *ucr, overlap_region_alloc *overlap_list, Candidates_list *cl, double bw_thres, int max_n_chain, int keep_whole_chain) { extern void *ha_flt_tab; extern ha_pt_t *ha_idx; uint32_t i; uint64_t k, l; // prepare clear_Candidates_list(cl); clear_overlap_region_alloc(overlap_list); recover_UC_Read(ucr, &R_INF, rid); ab->mz.n = 0, ab->n_a = 0; // get the list of anchors ha_sketch(ucr->seq, ucr->length, asm_opt.mz_win, asm_opt.k_mer_length, 0, !asm_opt.no_HPC, &ab->mz, ha_flt_tab); if (ab->mz.m > ab->old_mz_m) { ab->old_mz_m = ab->mz.m; REALLOC(ab->seed, ab->old_mz_m); } for (i = 0, ab->n_a = 0; i < ab->mz.n; ++i) { ab->seed[i].a = ha_pt_get(ha_idx, ab->mz.a[i].x, &ab->seed[i].n); ab->n_a += ab->seed[i].n; } if (ab->n_a > ab->m_a) { ab->m_a = ab->n_a; kroundup64(ab->m_a); REALLOC(ab->a, ab->m_a); } for (i = 0, k = 0; i < ab->mz.n; ++i) { int j; ha_mz1_t *z = &ab->mz.a[i]; seed1_t *s = &ab->seed[i]; for (j = 0; j < s->n; ++j) { const ha_idxpos_t *y = &s->a[j]; anchor1_t *an = &ab->a[k++]; uint8_t rev = z->rev == y->rev? 0 : 1; an->other_off = y->pos; an->self_off = rev? ucr->length - 1 - (z->pos + 1 - z->span) : z->pos; an->srt = (uint64_t)y->rid<<33 | (uint64_t)rev<<32 | an->other_off; } } // sort anchors radix_sort_ha_an1(ab->a, ab->a + ab->n_a); for (k = 1, l = 0; k <= ab->n_a; ++k) { if (k == ab->n_a || ab->a[k].srt != ab->a[l].srt) { if (k - l > 1) radix_sort_ha_an2(ab->a + l, ab->a + k); l = k; } } // copy over to _cl_ if (ab->m_a >= (uint64_t)cl->size) { cl->size = ab->m_a; REALLOC(cl->list, cl->size); } for (k = 0; k < ab->n_a; ++k) { k_mer_hit *p = &cl->list[k]; p->readID = ab->a[k].srt >> 33; p->strand = ab->a[k].srt >> 32 & 1; p->offset = ab->a[k].other_off; p->self_offset = ab->a[k].self_off; } cl->length = ab->n_a; calculate_overlap_region_by_chaining(cl, overlap_list, rid, ucr->length, &R_INF, bw_thres, keep_whole_chain); #if 0 if (overlap_list->length > 2000) { fprintf(stderr, "B\t%ld\t%ld\t%ld\n", (long)rid, (long)overlap_list->length, (long)Get_READ_LENGTH(R_INF, rid)); for (int i = 0; i < (int)overlap_list->length; ++i) { overlap_region *r = &overlap_list->list[i]; fprintf(stderr, "C\t%d\t%d\t%d\t%c\t%d\t%ld\t%d\t%d\t%c\t%d\n", (int)r->x_id, (int)r->x_pos_s, (int)r->x_pos_e, "+-"[r->x_pos_strand], (int)r->y_id, (long)Get_READ_LENGTH(R_INF, r->y_id), (int)r->y_pos_s, (int)r->y_pos_e, "+-"[r->y_pos_strand], (int)r->shared_seed); } } #endif if ((int)overlap_list->length > max_n_chain) { uint32_t n[2], s[2]; n[0] = n[1] = 0, s[0] = s[1] = 0; for (i = 0; i < (uint32_t)overlap_list->length; ++i) { const overlap_region *r = &overlap_list->list[i]; int dir = r->x_pos_s == 0? 0 : 1; ++n[dir]; if ((int)n[dir] == max_n_chain) s[dir] = r->shared_seed; } if (s[0] > 0 || s[1] > 0) { for (i = 0, k = 0; i < (uint32_t)overlap_list->length; ++i) { overlap_region *r = &overlap_list->list[i]; int dir = r->x_pos_s == 0? 0 : 1; if (r->shared_seed > s[dir]) { if ((uint32_t)k != i) { overlap_region t; t = overlap_list->list[k]; overlap_list->list[k] = overlap_list->list[i]; overlap_list->list[i] = t; } ++k; } } overlap_list->length = k; } } ks_introsort_or_xs(overlap_list->length, overlap_list->list); }