#include #include #include #include #include "kvec.h" #include "htab.h" #include "ksort.h" #include "Correct.h" #define MAX_HIGH_OCC 8 // TODO: don't hard code if we need to tune this parameter #define MAX_MAX_HIGH_OCC 16 #define GMC(a, x,y,xn) ((a)[(x)*(xn)+(y)]) static inline int mzcmp(const ha_mz1_t *a, const ha_mz1_t *b) { return a->rid < b->rid? -1 : a->rid > b->rid? 1 : ((a->x > b->x) - (a->x < b->x)); } #define mz_lt(a, b) (mzcmp(&(a), &(b)) < 0) KSORT_INIT(mz, ha_mz1_t, mz_lt) void debug_refine(ha_mz1_t *ma, uint64_t *mmt, int32_t sn, int32_t n, int32_t m, int32_t end) { uint64_t ks = end; int64_t t = 0, i, k, sp = -1, ep = -1, ovlp, tot = mmt[end]&0xffffffff, nt = 0;; while (ks != 0xffffffff) { i = ks/m; k = ks%m; ks = mmt[ks]>>32; if(ks == 0xffffffff || (int32_t)(ks/m) == (i-1)) { t++; ovlp = ((MIN(ep, (int64_t)ma[k].pos) >= MAX(sp, (int64_t)(ma[k].pos+1-ma[k].span)))? MIN(ep, (int64_t)ma[k].pos) - MAX(sp, (int64_t)(ma[k].pos+1-ma[k].span)) + 1:0); if(ovlp != 0) fprintf(stderr, "ERROR-OVLP\n"); if(sp == -1 || sp > (ma[k].pos+1-ma[k].span)) sp = ma[k].pos+1-ma[k].span; if(ep == -1 || ep < ma[k].pos) ep = ma[k].pos; nt += (ma[k].rid); } } if(t != sn) fprintf(stderr, "ERROR-TN, t: %ld, sn: %d\n", t, sn); if(nt != tot) fprintf(stderr, "ERROR-TOT, nt: %ld, tot: %ld\n", nt, tot); } void refine_select(ha_mz1_v *mz, int32_t sidx, int32_t eidx, int32_t sn, int32_t min_freq, st_mt_t *mm, int32_t *rsi, int32_t *rei) { int32_t n = sn, m = eidx + 1 - sidx, i, k, t, mk=-1; uint64_t ix, kx, ks; kv_resize(uint64_t, *mm, mm->n+n*m); ha_mz1_t *ma = mz->a + sidx; uint64_t *mmt = mm->a + mm->n; // fprintf(stderr, "[M::%s::] ==> +n: %d, m: %d, sn: %d, sidx: %d, eidx: %d\n", __func__, n, m, sn, sidx, eidx); for (i = 0; i < n; i++) ///how many selected minimizers { for (k = 0, mk = -1; k < m; k++) ///how many minimizers in total { if((int32_t)(ma[k].rid) 0) { for (t = k-1; t >= 0 && (ma[t].pos >= ks||(int32_t)(ma[t].rid)0?(i-1)*m+t:0xffffffff)<<32; else if(ks == kx) ks |= (uint64_t)(mk>=0?i*m+mk:0xffffffff)<<32; GMC(mmt, i,k,m) = ks; mk = k; } } // fprintf(stderr, "[M::%s::] ==> ++n: %d, m: %d, sn: %d, sidx: %d, eidx: %d\n", __func__, n, m, sn, sidx, eidx); ks = (n-1)*m + mk; ix = (uint64_t)-1; kx = 0; while (ks != 0xffffffff) { i = ks/m; k = ks%m; ks = mmt[ks]>>32; // fprintf(stderr, "i: %d, k: %d, ks: %lu\n", i, k, ks); if(ks == 0xffffffff || (int32_t)(ks/m) == (i-1)) { mm->a[sidx+k] = 1; ix = MIN((uint64_t)k, ix); kx = MAX((uint64_t)k, kx); } } ///debug // debug_refine(ma, mmt, sn, n, m, (n-1)*m + mk); if(rsi) (*rsi) = ix + sidx; if(rei) (*rei) = kx + sidx; } void refine_sketch(ha_mz1_v *p, ha_pt_t *pt, int32_t rlen, int32_t dp_min_len, float er, int32_t min_freq, st_mt_t *mt) { // fprintf(stderr, "[M::%s::] ==> #########10#########, rlen: %d\n", __func__, rlen); int32_t i, n = p->n, bd, len = MIN(rlen, dp_min_len), sublen, cnt, ei, li, ri; int32_t sn = len*er + 1; kv_resize(uint64_t, *mt, (int64_t)p->n); mt->n = p->n; memset(mt->a, 0, sizeof(uint64_t)*p->n); for (i = 0; i < n; i++) p->a[i].rid = ha_pt_cnt(pt, p->a[i].x); for (i = cnt = 0, bd = -1, ei = -1; i < n; i++) { if((int32_t)(p->a[i].rid)a[i].pos + 1; if(sublen > len) break; else ei = i; if((int32_t)(p->a[i].pos + 1 - p->a[i].span) > bd) { bd = p->a[i].pos; cnt++; } } // fprintf(stderr, "[M::%s::] ==> +cnt: %d, sn: %d, ei: %d, n: %d\n", __func__, cnt, sn, ei, n); if(cnt >= sn) refine_select(p, 0, ei, sn, min_freq, mt, NULL, &li); else { li = i-1; for (i = 0; i <= li; i++) mt->a[i] = 1; } if(len < rlen) { for (i = n-1, cnt = 0, bd = rlen+1, ei = -1; i >= 0; i--) { if((int32_t)(p->a[i].rid)a[i].pos + 1 - p->a[i].span); if(sublen > len) break; else ei = i; if((int32_t)(p->a[i].pos) < bd) { bd = p->a[i].pos + 1 - p->a[i].span; cnt++; } } // fprintf(stderr, "[M::%s::] ==> -cnt: %d, sn: %d, ei: %d, n: %d\n", __func__, cnt, sn, ei, n); if(cnt >= sn) refine_select(p, ei, n-1, sn, min_freq, mt, &ri, NULL); else { ri = i+1; for (i = ri; i <= n-1; i++) mt->a[i] = 1; } // fprintf(stderr, "[M::%s::] ==> --cnt: %d, sn: %d, ei: %d, n: %d\n", __func__, cnt, sn, ei, n); if(ri - li >= 2) { li++; ri--; sn = (p->a[ri].pos - p->a[li].pos + p->a[li].span)*er + 1; for (i = li, cnt = 0, bd = -1; i <= ri; i++) { if((int32_t)(p->a[i].rid)a[i].pos + 1 - p->a[i].span) > bd) { bd = p->a[i].pos; cnt++; if(cnt >= sn) break; } } if(cnt >= sn) refine_select(p, li, ri, sn, min_freq, mt, NULL, NULL); else for (i = li; i <= ri; i++) mt->a[i] = 1; } } // fprintf(stderr, "[M::%s::] ==> #########20#########, p->n: %u, n: %d\n", __func__, p->n, n); for (i = sn = 0; i < n; i++) { if(mt->a[i]) { p->a[sn] = p->a[i]; sn++; } } // if(p->n != sn) fprintf(stderr, "[M::%s::] ==> #########21#########, p->n: %u, sn: %d\n", __func__, p->n, sn); p->n = sn; } inline int hf_dp(ha_mz1_v *mz, int32_t sidx, int32_t eidx, int32_t sn, int32_t min_freq, st_mt_t *mm, int32_t *rsi, int32_t *rei) { return 0; } inline void hf_select(ha_mz1_v *p, int32_t si, int32_t ei, int32_t n, int32_t len, int32_t sample_dist, ha_mz1_t *b, int32_t force) { if(ei - si <= 1) return; int32_t ps = si < 0? 0 : p->a[si].pos; int32_t pe = ei == n? len : p->a[ei].pos; int32_t j, k, st = si + 1, en = ei; int32_t max_high_occ = (int32_t)((double)(pe - ps) / sample_dist + .499); if (max_high_occ > MAX_MAX_HIGH_OCC) max_high_occ = MAX_MAX_HIGH_OCC; for (j = st, k = 0; j < en && k < max_high_occ; ++j, ++k) b[k] = p->a[j], b[k].pos = j; // b[].pos keeps the index in p->a[] ks_heapmake_mz(k, b); // initialize the binomial heap for (; j < en; ++j) { // if there are more, choose top max_high_occ if (mz_lt(p->a[j], b[0])) { // then update the heap b[0] = p->a[j], b[0].pos = j; ks_heapdown_mz(0, k, b); } } //ks_heapsort_mz(k, b); // sorting is not needed for now for (j = 0; j < k; ++j) if (b[j].rid < pe - ps || force) p->a[b[j].pos].rid = 0; } void select_mz(ha_mz1_v *p, int len, int sample_dist, int32_t dp_min_len) { // for high-occ minimizers, choose up to max_high_occ in each high-occ streak int32_t i, last0 = -1, n = (int32_t)p->n, m = 0, nw[2], min_len; ha_mz1_t b[MAX_MAX_HIGH_OCC]; // this is to avoid a heap allocation if (n == 0 || n == 1) return; assert(n < 1<<27); // 27 is the number of bits for ha_mz1_t::pos; this should be safe as there are more bases than minimizers for (i = 0; i < n; ++i) if (p->a[i].rid != 0) ++m; if (m == 0) return; // no high-frequency k-mers; do nothing for (i = 0; i <= n; ++i) { if (i == n || p->a[i].rid == 0) { if (i - last0 > 1) { hf_select(p, last0, i, n, len, sample_dist, b, 0); // int32_t ps = last0 < 0? 0 : p->a[last0].pos; // int32_t pe = i == n? len : p->a[i].pos; // int32_t j, k, st = last0 + 1, en = i; // int32_t max_high_occ = (int32_t)((double)(pe - ps) / sample_dist + .499); // if (max_high_occ > MAX_MAX_HIGH_OCC) // max_high_occ = MAX_MAX_HIGH_OCC; // for (j = st, k = 0; j < en && k < max_high_occ; ++j, ++k) // b[k] = p->a[j], b[k].pos = j; // b[].pos keeps the index in p->a[] // ks_heapmake_mz(k, b); // initialize the binomial heap // for (; j < en; ++j) { // if there are more, choose top max_high_occ // if (mz_lt(p->a[j], b[0])) { // then update the heap // b[0] = p->a[j], b[0].pos = j; // ks_heapdown_mz(0, k, b); // } // } // //ks_heapsort_mz(k, b); // sorting is not needed for now // for (j = 0; j < k; ++j) // if (b[j].rid < pe - ps) // p->a[b[j].pos].rid = 0; } last0 = i; } } min_len = MAX(dp_min_len, (p->a[0].pos+1)+sample_dist); for (i = 0, nw[0] = nw[1] = 0; i < n; i++) { nw[(p->a[i].rid!=0)]++; if((p->a[i].pos + 1) > min_len) break; } if(nw[0]==0 && nw[1]>0) hf_select(p, -1, i, n, len, sample_dist, b, 1); min_len = MAX(dp_min_len, (len - (p->a[n-1].pos + 1 - p->a[n-1].span))+sample_dist); for (i = n-1, nw[0] = nw[1] = 0; i >= 0; i--) { nw[(p->a[i].rid!=0)]++; if((len - (p->a[i].pos + 1 - p->a[i].span)) > min_len) break; } if(nw[0]==0 && nw[1]>0) hf_select(p, i, n, n, len, sample_dist, b, 1); for (i = n = 0; i < (int32_t)p->n; ++i) // squeeze out filtered minimizers if (p->a[i].rid == 0) p->a[n++] = p->a[i]; p->n = n; } static inline int mzcmp_l(const ha_mz1_v *p, int32_t ai, int32_t bi) { if(ai >= 0 && bi >= 0){ ha_mz1_t *a = &(p->a[ai]), *b = &(p->a[bi]); if(a->rid > 0 && b->rid > 0) return mzcmp(a, b); return (a->rid == 0) - (b->rid == 0); } return (ai < 0) - (bi < 0); } #define GL(x, i) ((int64_t)((uint32_t)((x).a[(i)]))) #define A_M(p, i) ((i) >= 0 && (p).a[(i)].rid > 0) int32_t qfw(ha_mz1_v *p, st_mt_t *mt, int32_t n, int32_t tot_l, int32_t ws, int32_t i, int32_t *mi) { int32_t m, si; for (si = i, (*mi) = -1; i < n; i++){ if(GL(*mt, i) >= ws || (i+1 < n && GL(*mt, i) < ws && GL(*mt, i+1) > ws) || (i+1 == n && tot_l >= ws && GL(*mt, i) < ws)){ for (m = si; m <= i; m++){ if(!A_M(*p, m)) continue; if(mzcmp_l(p, *mi, m) >= 0) (*mi) = m; } if((*mi) >= 0 && A_M(*p, *mi)){ for (m = si; m <= i; m++){ if(!A_M(*p, m)) continue; if(mzcmp_l(p, *mi, m) == 0) mt->a[m] |= 0x100000000; } } break; } } return i; } void dbg_boundary(ha_mz1_v *p, st_mt_t *mt, int32_t w, int32_t k, int32_t tot_l) { if(tot_l < w + k -1) return; int32_t i, m, n = p->n, s, a; for (i = 0; i < n; i++){ if(GL(*mt, i) >= w+k-1){ for (m = s = a = 0; m <= i; m++){ if(!A_M(*p, m)) continue; if(GL(*mt, m) <= w+k-1){ a++; if(mt->a[m]&0x100000000) s++; } } if(a > 0 && s == 0){ fprintf(stderr, "\nERROR1, s: %d, n: %d, tot_l: %d, end_l: %ld\n", s, n, tot_l, GL(*mt, i)); for (m = s = a = 0; m <= i; m++){ if(!A_M(*p, m)) continue; if(GL(*mt, m) <= w+k-1){ fprintf(stderr, "lp: %ld\n", GL(*mt, m)); a++; if(mt->a[m]&0x100000000) s++; } } } break; } } if(i == n){ for (m = s = a = 0; m < n; m++){ if(!A_M(*p, m)) continue; if(GL(*mt, m) <= w+k-1){ a++; if(mt->a[m]&0x100000000) s++; } } if(a > 0 && s == 0) fprintf(stderr, "ERROR2\n"); } for (i = n-1; i >= 0; i--) { if (GL(*mt, i) + w <= tot_l + 1) { for (m = i, s = a = 0; m < n; m++){ if(!A_M(*p, m)) continue; if(GL(*mt, m) + w >= tot_l + 1){ a++; if(mt->a[m]&0x100000000) s++; } } if(a > 0 && s == 0) { fprintf(stderr, "\nERROR3, s: %d, n: %d, tot_l: %d, end_l: %ld\n", s, n, tot_l, GL(*mt, i)); for (m = i, s = a = 0; m < n; m++){ if(!A_M(*p, m)) continue; if(GL(*mt, m) + w >= tot_l + 1){ fprintf(stderr, "lp: %ld\n", GL(*mt, m)); a++; if(mt->a[m]&0x100000000) s++; } } } break; } } if(i < 0){ for (m = s = a = 0; m < n; m++){ if(!A_M(*p, m)) continue; if(GL(*mt, m) + w >= tot_l + 1){ a++; if(mt->a[m]&0x100000000) s++; } } if(a > 0 && s == 0) fprintf(stderr, "ERROR4\n"); } } static void select_mz_h(ha_mz1_v *p, st_mt_t *mt, int len, int sample_dist, int32_t w, int32_t k, int32_t tot_l) { // for high-occ minimizers, choose up to max_high_occ in each high-occ streak int32_t i, mi = -1, si, last0 = -1, n = (int32_t)p->n, m = 0, ws = w + k - 1; if (n == 0) return; assert(n < 1<<27); // 27 is the number of bits for ha_mz1_t::pos; this should be safe as there are more bases than minimizers for (i = m = 0, last0 = -1; i <= n; ++i) { if (i == n || p->a[i].rid == 0) { if (i - last0 > 1) { int32_t ps = last0 < 0? 0 : p->a[last0].pos; int32_t pe = i == n? len : p->a[i].pos; if(((int32_t)((double)(pe - ps) / sample_dist + .499)) > 0){ last0 = -2; m++; break; } } last0 = i; } } if (m == 0) return; // no high-frequency k-mers; do nothing if(last0 >= -1) goto ff; i = 0; i = qfw(p, mt, n, tot_l, ws, i, &mi); if(i == n) goto ff; for (si = 0, i++; i < n; i++){ for (; si < i; si++){ if(GL(*mt, si) + w > GL(*mt, i)) break; } // a new minimum; then write the old min if(mzcmp_l(p, i, mi) <= 0) { if(A_M(*p, mi)) mt->a[mi] |= 0x100000000; mi = i; }// old min has moved outside the window else if(si > mi){ if(A_M(*p, mi)) mt->a[mi] |= 0x100000000; for (m = si, mi = -1; m <= i; m++){ if(mzcmp_l(p, mi, m) >= 0) mi = m; } if(A_M(*p, mi)){ for (m = si; m <= i; m++){ if(!A_M(*p, m)) continue; if(mzcmp_l(p, mi, m) == 0) mt->a[m] |= 0x100000000; } } } } if(A_M(*p, mi)) mt->a[mi] |= 0x100000000; for (i = n - 1; si < n && GL(*mt, si) + w <= tot_l + 1; si++){ if(si > mi){ if(A_M(*p, mi)) mt->a[mi] |= 0x100000000; for (m = si, mi = -1; m <= i; m++){ if(mzcmp_l(p, mi, m) >= 0) mi = m; } if(A_M(*p, mi)){ for (m = si; m <= i; m++){ if(!A_M(*p, m)) continue; if(mzcmp_l(p, mi, m) == 0) mt->a[m] |= 0x100000000; } } } } /** dbg_boundary(p, mt, w, k, tot_l); fprintf(stderr, "\n"); for (i = 0; i < (int32_t)p->n; ++i){ if(p->a[i].rid == 0) continue; fprintf(stderr, "%cl: %u, pos: %lu, cnt: %lu, key: %lu, i: %d\n", "+-"[!!(mt->a[i]&0x100000000)], (uint32_t)mt->a[i], p->a[i].pos, p->a[i].rid, p->a[i].x, i); // if (mt->a[i]&0x100000000){ // fprintf(stderr, "+l: %u, pos: %lu, cnt: %lu\n", (uint32_t)mt->a[i], p->a[i].pos, p->a[i].rid); // } } **/ ha_mz1_t b[MAX_MAX_HIGH_OCC]; for (i = 0, last0 = -1; i <= n; ++i) { if (i == n || p->a[i].rid == 0) { if (i - last0 > 1) { int32_t ps = last0 < 0? 0 : p->a[last0].pos; int32_t pe = i == n? len : p->a[i].pos; if(((int32_t)((double)(pe - ps) / sample_dist + .499)) > 0){ for (m = last0 + 1, mi = 0; m < i; ++m){ if(mt->a[m]&0x100000000) p->a[m].rid = 0, mi++; } if(mi == 0) hf_select(p, last0, i, n, len, sample_dist, b, 0); } } last0 = i; } } ff: for (i = n = 0; i < (int32_t)p->n; ++i) // squeeze out filtered minimizers if (p->a[i].rid == 0) p->a[n++] = p->a[i]; p->n = n; } void debug_pl(const char *str, int len, int w, int k, int is_hpc, ha_mz1_v *p, const void *hf, st_mt_t *mt) { int i, l, dbi, dbcnt = 0, kmer_span = 0; tiny_queue_t tq; memset(&tq, 0, sizeof(tiny_queue_t)); uint64_t shift1 = k - 1, mask = (1ULL< k) kmer_span -= tq_shift(&tq); } else kmer_span = l + 1 < k? l + 1 : k; ///kmer_span should be used for HPC k-mer ///non-HPC k-mer, kmer_span should be k ///kmer_span is used to calculate anchor pos on reverse complementary strand kmer[0] = (kmer[0] << 1 | (c&1)) & mask; // forward k-mer kmer[1] = (kmer[1] << 1 | (c>>1)) & mask; kmer[2] = kmer[2] >> 1 | (uint64_t)(1 - (c&1)) << shift1; // reverse k-mer kmer[3] = kmer[3] >> 1 | (uint64_t)(1 - (c>>1)) << shift1; if (kmer[1] == kmer[3]) continue; // skip "symmetric k-mers" as we don't know it strand z = kmer[1] < kmer[3]? 0 : 1; // strand ++l; if (l >= k && kmer_span < 256) { uint64_t y; int32_t cnt; y = yak_hash64_64(kmer[z<<1|0]) + yak_hash64_64(kmer[z<<1|1]); cnt = hf? ha_ft_cnt(hf, y) : 0; for (dbi = 0; dbi < mt->n; dbi++) { if(p->a[dbi].x == y && p->a[dbi].rid == cnt && p->a[dbi].pos == i && p->a[dbi].rev == z && p->a[dbi].span == kmer_span) { if(l != (int)mt->a[dbi]) fprintf(stderr, "ERROR\n"); dbcnt++; } } } } else l = 0, tq.count = tq.front = 0, kmer_span = 0; } if(dbcnt != mt->n) fprintf(stderr, "ERROR\n"); if(mt->n != (int)p->n) fprintf(stderr, "ERROR\n"); for (dbi = 1; dbi < mt->n; dbi++) { if(p->a[dbi].pos <= p->a[dbi-1].pos || (int)mt->a[dbi] <= (int)mt->a[dbi-1]) { fprintf(stderr, "ERROR\n"); } } } /** * Find symmetric (w,k)-minimizers on a DNA sequence * * @param str DNA sequence * @param len length of $str * @param w find a minimizer for every $w consecutive k-mers * @param k k-mer size * @param rid reference ID; will be copied to the output $p array * @param is_hpc homopolymer-compressed or not * @param p minimizers */ void ha_sketch(const char *str, int len, int w, int k, uint32_t rid, int is_hpc, ha_mz1_v *p, const void *hf, int sample_dist, kvec_t_u8_warp* k_flag, kvec_t_u64_warp* dbg_ct, ha_pt_t *pt, int min_freq, int32_t dp_min_len, float dp_e, st_mt_t *mt, int32_t ws) { ///in default, w = 51, k = 51, is_hpc = 1 /** uint64_t x; uint64_t rid:28, pos:27, rev:1, span:8; **/ extern void *ha_ct_table; static const ha_mz1_t dummy = { UINT64_MAX, (1<<28) - 1, 0, 0, 0}; uint64_t shift1 = k - 1, mask = (1ULL< 0 && len < 1<<27 && rid < 1<<28 && (w > 0 && w < 256) && (k > 0 && k <= 63)); if (dbg_ct != NULL) dbg_ct->a.n = 0; if (k_flag != NULL) { kv_resize(uint8_t, k_flag->a, (uint64_t)len); k_flag->a.n = len; memset(k_flag->a.a, 0, k_flag->a.n); } memset(buf, 0xff, w * sizeof(ha_mz1_t)); memset(&tq, 0, sizeof(tiny_queue_t)); ///len/w is the evaluated minimizer numbers kv_resize(ha_mz1_t, *p, p->n + len/w); kv_resize(uint64_t, *mt, (int64_t)p->m); mt->n = p->n; for (i = l = tl = buf_pos = min_pos = 0; i < len; ++i) { int c = seq_nt4_table[(uint8_t)str[i]]; ha_mz1_t info = dummy; if (c < 4) { // not an ambiguous base int z; if (is_hpc) { int skip_len = 1; if (i + 1 < len && seq_nt4_table[(uint8_t)str[i + 1]] == c) { for (skip_len = 2; i + skip_len < len; ++skip_len) if (seq_nt4_table[(uint8_t)str[i + skip_len]] != c) break; i += skip_len - 1; // put $i at the end of the current homopolymer run } tq_push(&tq, skip_len); kmer_span += skip_len; ///how many bases that are covered by this HPC k-mer ///kmer_span includes at most k HPC elements if (tq.count > k) kmer_span -= tq_shift(&tq); } else kmer_span = l + 1 < k? l + 1 : k; ///kmer_span should be used for HPC k-mer ///non-HPC k-mer, kmer_span should be k ///kmer_span is used to calculate anchor pos on reverse complementary strand if (k_flag != NULL) k_flag->a.a[i] = 1;///lable all useful base, which are not ignored by HPC kmer[0] = (kmer[0] << 1 | (c&1)) & mask; // forward k-mer kmer[1] = (kmer[1] << 1 | (c>>1)) & mask; kmer[2] = kmer[2] >> 1 | (uint64_t)(1 - (c&1)) << shift1; // reverse k-mer kmer[3] = kmer[3] >> 1 | (uint64_t)(1 - (c>>1)) << shift1; if (kmer[1] == kmer[3]) continue; // skip "symmetric k-mers" as we don't know it strand z = kmer[1] < kmer[3]? 0 : 1; // strand ++l; tl++; if (l >= k && kmer_span < 256) { uint64_t y; int32_t cnt, filtered; y = yak_hash64_64(kmer[z<<1|0]) + yak_hash64_64(kmer[z<<1|1]); cnt = hf? ha_ft_cnt(hf, y) : 0; filtered = (cnt >= 1<<28); if (dbg_ct != NULL) kv_push(uint64_t, dbg_ct->a, ((((uint64_t)(query_ct_index(ha_ct_table, y))<<1)|filtered)<<32)|(uint64_t)(i)); if (!filtered) info.x = y, info.rid = cnt, info.pos = i, info.rev = z, info.span = kmer_span; // initially ha_mz1_t::rid keeps the k-mer count if (k_flag != NULL) k_flag->a.a[i]++; if (k_flag != NULL && filtered > 0) k_flag->a.a[i]++; } } else l = 0, tq.count = tq.front = 0, kmer_span = 0; buf[buf_pos] = info; // need to do this here as appropriate buf_pos and buf[buf_pos] are needed below buf_p[buf_pos] = l; if (l == w + k - 1 && min.x != UINT64_MAX) { // special case for the first window - because identical k-mers are not stored yet for (j = buf_pos + 1; j < w; ++j){ if (mzcmp(&min, &buf[j]) == 0 && buf[j].pos != min.pos){ kv_push(ha_mz1_t, *p, buf[j]); kv_push(uint64_t, *mt, buf_p[j]); } } for (j = 0; j < buf_pos; ++j){ if (mzcmp(&min, &buf[j]) == 0 && buf[j].pos != min.pos){ kv_push(ha_mz1_t, *p, buf[j]); kv_push(uint64_t, *mt, buf_p[j]); } } } /** * There are three cases: * 1. info.x <= min.x, means info is a new minimizer * 2. info.x > min.x, info is not a new minimizer * (1) buf_pos != min_pos, do nothing * (2) buf_pos == min_pos, means current minimizer has moved outside the window * **/ ///three cases: 1. if (mzcmp(&min, &info) >= 0) { // a new minimum; then write the old min if (l >= w + k && min.x != UINT64_MAX){ kv_push(ha_mz1_t, *p, min); kv_push(uint64_t, *mt, min_s); } min = info, min_pos = buf_pos, min_s = buf_p[buf_pos]; } else if (buf_pos == min_pos) { // old min has moved outside the window if (l >= w + k - 1 && min.x != UINT64_MAX){ kv_push(ha_mz1_t, *p, min); kv_push(uint64_t, *mt, min_s); } ///buf_pos == min_pos, means current minimizer has moved outside the window ///so for now we need to find a new minimizer at the current window (w k-mers) for (j = buf_pos + 1, min = dummy; j < w; ++j) // the two loops are necessary when there are identical k-mers if (mzcmp(&min, &buf[j]) >= 0) min = buf[j], min_pos = j, min_s = buf_p[j]; // >= is important s.t. min is always the closest k-mer for (j = 0; j <= buf_pos; ++j) if (mzcmp(&min, &buf[j]) >= 0) min = buf[j], min_pos = j, min_s = buf_p[j]; if (l >= w + k - 1 && min.x != UINT64_MAX) { // write identical k-mers for (j = buf_pos + 1; j < w; ++j) // these two loops make sure the output is sorted if (mzcmp(&min, &buf[j]) == 0 && min.pos != buf[j].pos){ kv_push(ha_mz1_t, *p, buf[j]); kv_push(uint64_t, *mt, buf_p[j]); } for (j = 0; j <= buf_pos; ++j) if (mzcmp(&min, &buf[j]) == 0 && min.pos != buf[j].pos){ kv_push(ha_mz1_t, *p, buf[j]); kv_push(uint64_t, *mt, buf_p[j]); } } } if (++buf_pos == w) buf_pos = 0; } if (min.x != UINT64_MAX){ kv_push(ha_mz1_t, *p, min); kv_push(uint64_t, *mt, min_s); } // debug_pl(str, len, w, k, is_hpc, p, hf, mt); // if (sample_dist > w) select_mz(p, len, MAX_HIGH_OCC, dp_min_len); select_mz_h(p, mt, len, sample_dist, ws, k, tl); if (dp_min_len > 0 && pt && mt) refine_sketch(p, pt, len, dp_min_len, dp_e, min_freq, mt); for (i = 0; i < (int)p->n; ++i) // populate .rid as this was keeping counts p->a[i].rid = rid; } void ha_sketch_worse(const char *str, int len, int w, int k, uint32_t rid, int is_hpc, ha_mz1_v *p, const void *hf, int sample_dist, kvec_t_u8_warp* k_flag, kvec_t_u64_warp* dbg_ct, ha_pt_t *pt, int min_freq, int32_t dp_min_len, float dp_e, st_mt_t *mt) { ///in default, w = 51, k = 51, is_hpc = 1 /** uint64_t x; uint64_t rid:28, pos:27, rev:1, span:8; **/ extern void *ha_ct_table; static const ha_mz1_t dummy = { UINT64_MAX, (1<<28) - 1, 0, 0 }; uint64_t shift1 = k - 1, mask = (1ULL< 0 && len < 1<<27 && rid < 1<<28 && (w > 0 && w < 256) && (k > 0 && k <= 63)); if (dbg_ct != NULL) dbg_ct->a.n = 0; if (k_flag != NULL) { kv_resize(uint8_t, k_flag->a, (uint64_t)len); k_flag->a.n = len; memset(k_flag->a.a, 0, k_flag->a.n); } memset(buf, 0xff, w * sizeof(ha_mz1_t)); memset(&tq, 0, sizeof(tiny_queue_t)); ///len/w is the evaluated minimizer numbers kv_resize(ha_mz1_t, *p, p->n + len/w); for (i = l = buf_pos = min_pos = 0; i < len; ++i) { int c = seq_nt4_table[(uint8_t)str[i]]; ha_mz1_t info = dummy; if (c < 4) { // not an ambiguous base int z; if (is_hpc) { int skip_len = 1; if (i + 1 < len && seq_nt4_table[(uint8_t)str[i + 1]] == c) { for (skip_len = 2; i + skip_len < len; ++skip_len) if (seq_nt4_table[(uint8_t)str[i + skip_len]] != c) break; i += skip_len - 1; // put $i at the end of the current homopolymer run } tq_push(&tq, skip_len); kmer_span += skip_len; ///how many bases that are covered by this HPC k-mer ///kmer_span includes at most k HPC elements if (tq.count > k) kmer_span -= tq_shift(&tq); } else kmer_span = l + 1 < k? l + 1 : k; ///kmer_span should be used for HPC k-mer ///non-HPC k-mer, kmer_span should be k ///kmer_span is used to calculate anchor pos on reverse complementary strand if (k_flag != NULL) k_flag->a.a[i] = 1;///lable all useful base, which are not ignored by HPC kmer[0] = (kmer[0] << 1 | (c&1)) & mask; // forward k-mer kmer[1] = (kmer[1] << 1 | (c>>1)) & mask; kmer[2] = kmer[2] >> 1 | (uint64_t)(1 - (c&1)) << shift1; // reverse k-mer kmer[3] = kmer[3] >> 1 | (uint64_t)(1 - (c>>1)) << shift1; if (kmer[1] == kmer[3]) continue; // skip "symmetric k-mers" as we don't know it strand z = kmer[1] < kmer[3]? 0 : 1; // strand ++l; if (l >= k && kmer_span < 256) { uint64_t y; int32_t cnt, filtered; y = yak_hash64_64(kmer[z<<1|0]) + yak_hash64_64(kmer[z<<1|1]); cnt = hf? ha_ft_cnt(hf, y) : 0; filtered = (cnt >= 1<<28); if (dbg_ct != NULL) kv_push(uint64_t, dbg_ct->a, ((((uint64_t)(query_ct_index(ha_ct_table, y))<<1)|filtered)<<32)|(uint64_t)(i)); if (!filtered) info.x = y, info.rid = pt? ha_pt_cnt(pt, y):cnt, info.pos = i, info.rev = z, info.span = kmer_span; // initially ha_mz1_t::rid keeps the k-mer count if (k_flag != NULL) k_flag->a.a[i]++; if (k_flag != NULL && filtered > 0) k_flag->a.a[i]++; } } else l = 0, tq.count = tq.front = 0, kmer_span = 0; //for non-HPC k-mer, l = i; but for HPC k-mer, l is always less than i //i is the real base iterator, while l is the HPC base iterator //only if l >= k, info is a useful minimizer (ha_mz1_t.x != UINT64_MAX) //but even if l < k, infor is still stored into buf buf[buf_pos] = info; // need to do this here as appropriate buf_pos and buf[buf_pos] are needed below if (l == w + k - 1 && min.x != UINT64_MAX) { // special case for the first window - because identical k-mers are not stored yet for (j = buf_pos + 1; j < w; ++j) if (mzcmp(&min, &buf[j]) == 0 && buf[j].pos != min.pos) kv_push(ha_mz1_t, *p, buf[j]); for (j = 0; j < buf_pos; ++j) if (mzcmp(&min, &buf[j]) == 0 && buf[j].pos != min.pos) kv_push(ha_mz1_t, *p, buf[j]); } /** * There are three cases: * 1. info.x <= min.x, means info is a new minimizer * 2. info.x > min.x, info is not a new minimizer * (1) buf_pos != min_pos, do nothing * (2) buf_pos == min_pos, means current minimizer has moved outside the window * **/ ///three cases: 1. if (info.x <= min.x) { // a new minimum; then write the old min if (l >= w + k && min.x != UINT64_MAX) kv_push(ha_mz1_t, *p, min); min = info, min_pos = buf_pos; } else if (buf_pos == min_pos) { // old min has moved outside the window if (l >= w + k - 1 && min.x != UINT64_MAX) kv_push(ha_mz1_t, *p, min); ///buf_pos == min_pos, means current minimizer has moved outside the window ///so for now we need to find a new minimizer at the current window (w k-mers) for (j = buf_pos + 1, min.x = UINT64_MAX; j < w; ++j) // the two loops are necessary when there are identical k-mers if (mzcmp(&min, &buf[j]) >= 0) min = buf[j], min_pos = j; // >= is important s.t. min is always the closest k-mer for (j = 0; j <= buf_pos; ++j) if (mzcmp(&min, &buf[j]) >= 0) min = buf[j], min_pos = j; if (l >= w + k - 1 && min.x != UINT64_MAX) { // write identical k-mers for (j = buf_pos + 1; j < w; ++j) // these two loops make sure the output is sorted if (mzcmp(&min, &buf[j]) == 0 && min.pos != buf[j].pos) kv_push(ha_mz1_t, *p, buf[j]); for (j = 0; j <= buf_pos; ++j) if (mzcmp(&min, &buf[j]) == 0 && min.pos != buf[j].pos) kv_push(ha_mz1_t, *p, buf[j]); } } if (++buf_pos == w) buf_pos = 0; } if (min.x != UINT64_MAX) kv_push(ha_mz1_t, *p, min); if (dp_min_len > 0 && pt && mt) refine_sketch(p, pt, len, dp_min_len, dp_e, min_freq, mt); // if (sample_dist > w) select_mz(p, len, MAX_HIGH_OCC); for (i = 0; i < (int)p->n; ++i) // populate .rid as this was keeping counts p->a[i].rid = rid; }