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r1275: renamed --jump-pass1 to --pass1
Also documented 2-pass
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2
main.c
2
main.c
@@ -84,7 +84,7 @@ static ko_longopt_t long_options[] = {
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{ "pairing", ko_required_argument, 359 },
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{ "jump-min-match", ko_required_argument, 360 },
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{ "write-junc", ko_no_argument, 361 },
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{ "jump-pass1", ko_required_argument, 362 },
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{ "pass1", ko_required_argument, 362 },
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{ "dbg-seed-occ", ko_no_argument, 501 },
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{ "help", ko_no_argument, 'h' },
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{ "max-intron-len", ko_required_argument, 'G' },
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@@ -5,7 +5,7 @@
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#include <stdio.h>
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#include <sys/types.h>
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#define MM_VERSION "2.28-r1274-dirty"
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#define MM_VERSION "2.28-r1275-dirty"
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#define MM_F_NO_DIAG (0x001LL) // no exact diagonal hit
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#define MM_F_NO_DUAL (0x002LL) // skip pairs where query name is lexicographically larger than target name
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27
minimap2.1
27
minimap2.1
@@ -1,4 +1,4 @@
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.TH minimap2 1 "6 April 2025" "minimap2-2.28-dirty (r1264)" "Bioinformatics tools"
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.TH minimap2 1 "13 April 2025" "minimap2-2.28-dirty (r1274)" "Bioinformatics tools"
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.SH NAME
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.PP
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minimap2 - mapping and alignment between collections of DNA sequences
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@@ -361,9 +361,9 @@ has no effect with the default
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Junctions used to extend alignment towards ends of reads [].
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.I FILE
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can be gene annotations in the BED12 format (aka 12-column BED), or intron
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positions in 5-column BED. BED12 file can be converted from GTF/GFF3 with
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`paftools.js gff2bed anno.gtf'.
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This option is intended for short RNA-seq reads, while
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positions in 5-column BED with the strand column required. BED12 file can be
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converted from GTF/GFF3 with `paftools.js gff2bed anno.gtf'. This option is
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intended for short RNA-seq reads, while
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.B --junc-bed
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for long noisy RNA-seq reads.
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.TP
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@@ -547,6 +547,25 @@ In SAM output, use soft clipping for supplementary alignments.
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.B --secondary-seq
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In SAM output, show query sequences for secondary alignments.
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.TP
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.B --write-junc
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Output splice junctions in 6-column BED: contig name, start, end,
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read name, score and strand. Score is the sum of donor and acceptor scores,
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where GT gets 3, GC gets 2 and AT gets 1 at donor sites,
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while AG gets 3 and AC gets 1 at acceptor sites.
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.TP
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.BI --pass1 \ FILE
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Junctions BED file outputted by
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.B --write-junc
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[]. Rows with scores lower than 5 are ignored. When both
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.B -j
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and
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.B --pass1
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are present, junctions in
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.B -j
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are preferred over in
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.BR --pass1
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when there is ambiguity.
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.TP
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.BI --seed \ INT
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Integer seed for randomizing equally best hits. Minimap2 hashes
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.I INT
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