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This was an oversight affecting v2.22+. The latest minimap2 ranks hits and estimates mapping quality with an adjusted alignment score (see the minimap2 update paper). This score however is not calculated when there is only one hit. As a result, the ms:i tag varies depends on other sequences in the reference genome, which is confusing. This change lets minimap2 to output the unadjusted score at ms:i. At present, the adjusted score is not outputted. Resolves #1146
37 KiB
37 KiB