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chhylp123
2022-10-01 23:42:38 -04:00
parent bbd3394b4a
commit 41437764f0
5 changed files with 88 additions and 41 deletions
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Assembly.cpp
-2
View File
@@ -468,7 +468,6 @@ ha_ovec_buf_t *ha_ovec_init(int is_final, int save_ov, int is_ug)
kv_init(b->k_flag.a);
kv_init(b->sp);
init_bit_extz_t(&(b->exz), 31);
init_bit_extz_t(&(b->exz1), 31);
if(!is_ug) b->ab = ha_abuf_init();
else b->abl = ha_abufl_init();
if (!b->is_final) {
@@ -498,7 +497,6 @@ void ha_ovec_destroy(ha_ovec_buf_t *b)
kv_destroy(b->k_flag.a);
kv_destroy(b->sp);
destroy_bit_extz_t(&(b->exz));
destroy_bit_extz_t(&(b->exz1));
if (!b->is_final) {
destory_Cigar_record(&b->cigar1);
destory_Graph(&b->POA_Graph);
Assembly.h
+1 -1
View File
@@ -40,7 +40,7 @@ typedef struct {
overlap_region tmp_region;
ma_utg_v *ua;
st_mt_t sp;
bit_extz_t exz, exz1;
bit_extz_t exz;
} ha_ovec_buf_t;
int ha_assemble(void);
Correct.cpp
+68 -33
View File
@@ -13575,7 +13575,7 @@ All_reads *rref, int64_t id)
} else if(rref) {
recover_UC_Read_sub_region(str, ss, sl, rev, rref, id);
}
return str;
return tu->seq;
} else {
return hpc_str(*hpc_g, id, rev) + s;
}
@@ -13637,6 +13637,28 @@ void cal_exz_semi(char *pstr, int32_t pn, char *tstr, int32_t tn, int32_t thre,
}
}
void ref_cigar_check(char* qstr, UC_Read *tu, const ul_idx_t *uref, hpc_t *hpc_g, All_reads *rref, int64_t id, int64_t rev, bit_extz_t *ez)
{
int64_t pts = -1, pte = -1, tl = ez->pe-ez->ps+1, ql = ez->te-ez->ts+1, bps, bts, k;
char *q, *t; bps = ez->ps; bts = ez->ts;
q = qstr + ez->ts;
t = retrieve_str_seq_exz(tu, ez->ps, tl, pts, pte-pts, rev, uref, hpc_g, rref, id);
ez->ps = ez->ts = 0;
if(!cigar_check(t, q, ez)){
fprintf(stderr, "[M::%s::] cigar_n::%d\n", __func__, (int32_t)ez->cigar.n);
for (k = 0; k < (int32_t)ez->cigar.n; k++) {
fprintf(stderr, "[M::%s::%ld] cigar_len::%u, c::%u\n", __func__, k,
ez->cigar.a[k]&(0x3fff), (ez->cigar.a[k]>>14));
}
fprintf(stderr, "[M::%s::l->%ld] s::%ld, pstr::%.*s\n", __func__, tl, bps, (int32_t)tl, t);
fprintf(stderr, "[M::%s::l->%ld] s::%ld, tstr::%.*s\n", __func__, ql, bts, (int32_t)ql, q);
exit(1);
}
ez->ps = bps; ez->ts = bts;
}
int64_t cal_exz_infi_adv(overlap_region *z, const ul_idx_t *uref, hpc_t *hpc_g, All_reads *rref,
bit_extz_t *exz, char* qstr, UC_Read *tu, int64_t qs, int64_t qe, int64_t ts, int64_t te,
int64_t *pts, int64_t *pte, int64_t thre, int64_t *pthre, int64_t q_tot_l, int64_t mode)
@@ -13677,12 +13699,16 @@ int64_t *pts, int64_t *pte, int64_t thre, int64_t *pthre, int64_t q_tot_l, int64
}
if(is_align(*exz)) {
// if(exz->err < 0) {
// fprintf(stderr, "\n[M::%s::ql::%ld] qs::%ld, qe::%ld, ts::%ld, te::%ld, mode::%ld, err::%d, thre::%d\n",
// __func__, ql, qs, qe, ts, te, mode, exz->err, exz->thre);
// fprintf(stderr, "[M::%s::] pstr::%.*s\n", __func__, (int32_t)tu->length, tu->seq);
// fprintf(stderr, "[M::%s::] tstr::%.*s\n", __func__, (int32_t)(qe-qs), qstr+qs);
// cigar_check(t_string, q_string, exz);
// if(mode == 1) {
// fprintf(stderr, "\n[M::%s::ql::%ld] qs::%ld, qe::%ld, ts::%ld, te::%ld, mode::%ld, err::%d, thre::%d, exz_q[%d, %d], exz_t[%d, %d]\n",
// __func__, ql, qs, qe, ts, te, mode, exz->err, exz->thre, exz->ts, exz->te, exz->ps, exz->pe);
// fprintf(stderr, "[M::%s::] pstr::%.*s\n", __func__, (int32_t)tl, t_string);
// fprintf(stderr, "[M::%s::] tstr::%.*s\n", __func__, (int32_t)ql, q_string);
// fprintf(stderr, "[M::%s::] exz->cigar.n::%d\n", __func__, (int32_t)exz->cigar.n);
// }
exz->ps += ts; exz->pe += ts;
exz->ts += qs; exz->te += qs;
return 1;
}
return 0;
@@ -13695,7 +13721,7 @@ int64_t cal_exact_exz(overlap_region *z, const ul_idx_t *uref, hpc_t *hpc_g, All
bit_extz_t *exz, char* qstr, UC_Read *tu, int64_t qs, int64_t qe, int64_t ts, int64_t te,
int64_t *pts, int64_t *pte, int64_t q_tot_l, int64_t mode)
{
clear_align(*exz); exz->thre = 0;
clear_align(*exz); exz->thre = 0; exz->cigar.n = 0;
int64_t ql, tl, t_tot_l = -1;
char *q_string, *t_string; int32_t rev = z->y_pos_strand, id = z->y_id; ql = qe - qs;
if(hpc_g) t_tot_l = hpc_len(*hpc_g, id);
@@ -13722,8 +13748,16 @@ int64_t *pts, int64_t *pte, int64_t q_tot_l, int64_t mode)
if(memcmp(q_string, t_string, ql)) return 0;
exz->err = 0; push_trace(&(exz->cigar), 0, ql);
exz->pl = tl; exz->ps = 0; exz->pe = tl;
exz->tl = ql; exz->ts = 0; exz->te = ql;
exz->pl = tl; exz->ps = 0; exz->pe = tl-1;
exz->tl = ql; exz->ts = 0; exz->te = ql-1;
// cigar_check(t_string, q_string, exz);
// if(!cigar_check(t_string, q_string, exz)) {
// fprintf(stderr, "[M::%s::] cigar_n::%d\n", __func__, (int32_t)exz->cigar.n);
// fprintf(stderr, "[M::%s::] pstr::%.*s\n", __func__, (int32_t)tl, t_string);
// fprintf(stderr, "[M::%s::] tstr::%.*s\n", __func__, (int32_t)ql, q_string);
// }
exz->ps += ts; exz->pe += ts;
exz->ts += qs; exz->te += qs;
return 1;
}
@@ -13746,6 +13780,7 @@ int64_t estimate_err)
// __func__, ql, qs, qe, ts, te, mode, estimate_err, e_rate, maxe);
if(ql <= 16) {
if(cal_exact_exz(z, uref, hpc_g, rref, exz, qstr, tu, qs, qe, ts, te, &pts, &pte, q_tot, mode)) {
// ref_cigar_check(qstr, tu, uref, hpc_g, rref, z->y_id, z->y_pos_strand, exz);
// fprintf(stderr, ", err::%d, thre::%d, scale::0(+)\n", exz->err, exz->thre);
return 1;
}
@@ -13754,6 +13789,7 @@ int64_t estimate_err)
if(ql <= maxl && (estimate_err>>1) <= maxe) {
thre = scale_ed_thre(estimate_err, maxe);
if(cal_exz_infi_adv(z, uref, hpc_g, rref, exz, qstr, tu, qs, qe, ts, te, &pts, &pte, thre, &pthre, q_tot, mode)) {
// ref_cigar_check(qstr, tu, uref, hpc_g, rref, z->y_id, z->y_pos_strand, exz);
// fprintf(stderr, ", err::%d, thre::%d, scale::%ld(+)\n", exz->err, exz->thre, thre);
return 1;
}
@@ -13761,6 +13797,7 @@ int64_t estimate_err)
thre0 = thre; thre = ql*e_rate; thre = scale_ed_thre(thre, maxe);
if(thre > thre0) {
if(cal_exz_infi_adv(z, uref, hpc_g, rref, exz, qstr, tu, qs, qe, ts, te, &pts, &pte, thre, &pthre, q_tot, mode)) {
// ref_cigar_check(qstr, tu, uref, hpc_g, rref, z->y_id, z->y_pos_strand, exz);
// fprintf(stderr, ", err::%d, thre::%d, scale::%ld(-)\n", exz->err, exz->thre, thre);
return 1;
}
@@ -13769,6 +13806,7 @@ int64_t estimate_err)
thre0 = thre; thre <<= 1; thre = scale_ed_thre(thre, maxe);
if(thre > thre0) {
if(cal_exz_infi_adv(z, uref, hpc_g, rref, exz, qstr, tu, qs, qe, ts, te, &pts, &pte, thre, &pthre, q_tot, mode)) {
// ref_cigar_check(qstr, tu, uref, hpc_g, rref, z->y_id, z->y_pos_strand, exz);
// fprintf(stderr, ", err::%d, thre::%d, scale::%ld(-)\n", exz->err, exz->thre, thre);
return 1;
}
@@ -13777,6 +13815,7 @@ int64_t estimate_err)
thre0 = thre; thre = ql*0.51; thre = scale_ed_thre(thre, maxe);
if(thre > thre0) {
if(cal_exz_infi_adv(z, uref, hpc_g, rref, exz, qstr, tu, qs, qe, ts, te, &pts, &pte, thre, &pthre, q_tot, mode)) {
// ref_cigar_check(qstr, tu, uref, hpc_g, rref, z->y_id, z->y_pos_strand, exz);
// fprintf(stderr, ", err::%d, thre::%d, scale::%ld(*)\n", exz->err, exz->thre, thre);
return 1;
}
@@ -13785,6 +13824,7 @@ int64_t estimate_err)
if(ql <= force_l) {
thre = maxe;
if(cal_exz_infi_adv(z, uref, hpc_g, rref, exz, qstr, tu, qs, qe, ts, te, &pts, &pte, thre, &pthre, q_tot, mode)) {
// ref_cigar_check(qstr, tu, uref, hpc_g, rref, z->y_id, z->y_pos_strand, exz);
// fprintf(stderr, ", err::%d, thre::%d, scale::%ld(*)\n", exz->err, exz->thre, thre);
return 1;
}
@@ -14068,12 +14108,12 @@ int64_t fusion_chain_ovlp(overlap_region *z, k_mer_hit *ch_a, int64_t ch_n, ul_o
// }
int64_t ovlp_base_aln_all(overlap_region *z, Chain_Data *dp, Candidates_list *cl, int64_t ch_tot_beg,
int64_t ch_tot_n, int64_t soff, int64_t eoff, const ul_idx_t *uref, hpc_t *hpc_g, All_reads *rref,
char* qstr, UC_Read *tu, ul_ov_t *ov, int64_t ql, int64_t tl, int64_t wl, bit_extz_t *exz, double e_rate)
int64_t ovlp_base_aln_all(overlap_region *z, Chain_Data *dp, k_mer_hit *ch_a, int64_t ch_n,
int64_t soff, int64_t eoff, const ul_idx_t *uref, hpc_t *hpc_g, All_reads *rref,
char* qstr, UC_Read *tu, ul_ov_t *ov, int64_t ql, int64_t tl, int64_t wl, bit_extz_t *exz,
double e_rate)
{
k_mer_hit *ch_a = cl->list + ch_tot_beg;
int64_t ch_n = ch_tot_n, ibeg, iend, i, l, mode, q[2], t[2], is_done;
int64_t ibeg, iend, i, l, mode, q[2], t[2], is_done;
ibeg = soff; iend = eoff;
for (l = ibeg, i = ibeg + 1; i <= iend; i++) {
l = i - 1;
@@ -14109,24 +14149,20 @@ char* qstr, UC_Read *tu, ul_ov_t *ov, int64_t ql, int64_t tl, int64_t wl, bit_ex
}
if(!is_done) {
// chain_win_aln(z, dp, cl, q[0], q[1], t[0], t[1], ql, tl, wl, exz);
ch_a = cl->list + ch_tot_beg;//update ch_a
}
}
return 0;
}
void ovlp_base_aln(overlap_region *z, Chain_Data *dp, Candidates_list *cl, int64_t ch_beg, int64_t ch_n,
void ovlp_base_aln(overlap_region *z, Chain_Data *dp, k_mer_hit *ch_a, int64_t ch_n,
ul_ov_t *ov, int64_t wl, const ul_idx_t *uref, hpc_t *hpc_g, All_reads *rref, char* qstr, UC_Read *tu,
bit_extz_t *exz, bit_extz_t *exz1, double e_rate, int64_t ql, int64_t tl, uint64_t rid)
bit_extz_t *exz, double e_rate, int64_t ql, int64_t tl, uint64_t rid)
{
int64_t ibeg, iend, i, l, mode, q[2], t[2], is_done;
k_mer_hit *ch_a = cl->list + ch_beg;
if(ov->qn == ((uint32_t)-1)) ibeg = -1;
else ibeg = ov->qn;
iend = ov->tn;
assert(iend>=ibeg+1);
exz1->err = 0; exz1->thre = 0;
exz1->ps = exz1->pe = (uint32_t)-1;
for (l = ibeg, i = ibeg + 1; i <= iend; i++) {
if(i == iend || is_pri_aln(ch_a[i])) {
@@ -14162,8 +14198,7 @@ bit_extz_t *exz, bit_extz_t *exz1, double e_rate, int64_t ql, int64_t tl, uint64
}
if(!is_done) {///postprocess
is_done = ovlp_base_aln_all(z, dp, cl, ch_beg, ch_n, l, i, uref, hpc_g, rref, qstr, tu, ov, ql, tl, wl, exz, e_rate);
ch_a = cl->list + ch_beg;///update ch_a
is_done = ovlp_base_aln_all(z, dp, ch_a, ch_n, l, i, uref, hpc_g, rref, qstr, tu, ov, ql, tl, wl, exz, e_rate);
}
l = i;
}
@@ -14171,21 +14206,20 @@ bit_extz_t *exz, bit_extz_t *exz1, double e_rate, int64_t ql, int64_t tl, uint64
}
void cigar_gen_by_chain_adv(overlap_region *z, Candidates_list *cl, int64_t ch_beg, int64_t ch_n,
void cigar_gen_by_chain_adv(overlap_region *z, k_mer_hit *ch_a, int64_t ch_n, Chain_Data *dp,
ul_ov_t *ov, int64_t on, uint64_t wl, const ul_idx_t *uref, hpc_t *hpc_g, All_reads *rref,
char* qstr, UC_Read *tu, bit_extz_t *exz, bit_extz_t *exz1, double e_rate, int64_t ql, uint64_t rid)
char* qstr, UC_Read *tu, bit_extz_t *exz, overlap_region *aux_o, double e_rate, int64_t ql, uint64_t rid)
{
if(on <= 0) return;
int64_t i, tl, id = z->y_id; k_mer_hit *ch_a; Chain_Data *dp;
int64_t i, tl, id = z->y_id;
if(hpc_g) tl = hpc_len(*hpc_g, id);
else if(uref) tl = uref->ug->u.a[id].len;
else tl = Get_READ_LENGTH((*rref), id);
dp = &(cl->chainDP); ch_a = cl->list + ch_beg;
on = fusion_chain_ovlp(z, ch_a, ch_n, ov, on, wl, ql, tl);
for (i = 0; i < on; i++) {
// ch_a = cl->list + ch_beg;
ovlp_base_aln(z, dp, cl, ch_beg, ch_n, &(ov[i]), wl, uref, hpc_g, rref, qstr, tu, exz, exz1, e_rate, ql, tl, rid);
ovlp_base_aln(z, dp, ch_a, ch_n, &(ov[i]), wl, uref, hpc_g, rref, qstr, tu, exz, e_rate, ql, tl, rid);
}
// for (i = 0; i < wn; i++) z->w_list.a[i].clen = 0;///clean cigar
@@ -14203,11 +14237,11 @@ char* qstr, UC_Read *tu, bit_extz_t *exz, bit_extz_t *exz1, double e_rate, int64
void ul_gap_filling_adv(overlap_region_alloc* ol, Candidates_list *cl, kv_ul_ov_t *aln, uint64_t wl,
const ul_idx_t *uref, hpc_t *hpc_g, All_reads *rref, char* qstr, UC_Read *tu, bit_extz_t *exz, bit_extz_t *exz1,
const ul_idx_t *uref, hpc_t *hpc_g, All_reads *rref, char* qstr, UC_Read *tu, bit_extz_t *exz, overlap_region *aux_o,
asg64_v* buf, asg64_v* iidx, double e_rate, int64_t ql, uint64_t rid, int64_t khit, int64_t base_chekc_k_hit,
int64_t max_lgap)
{
int64_t k, l, ch_n, a_n = aln->n; overlap_region *z;
int64_t k, l, ch_n, a_n = aln->n; overlap_region *z; k_mer_hit *ch_a;
// count_k_hits(rref, uref, qstr, tu, ol, cl, buf, khit, base_chekc_k_hit);
count_k_hits_adv(rref, uref, qstr, tu, ol, cl, buf, &(cl->chainDP), e_rate, khit, base_chekc_k_hit);
for (k = 1, l = 0; k <= a_n; k++) {
@@ -14215,9 +14249,9 @@ int64_t max_lgap)
z = &(ol->list[aln->a[l].qn]); assert(z->align_length == l);
ch_n = gen_cns_chain(z, cl, iidx, max_lgap, e_rate, 0);
if(ch_n) {
// ch_a = cl->list + cl->length;
ch_a = cl->list + cl->length;
// cigar_gen_by_chain(z, &(cl->chainDP), ch_a, ch_n, aln->a+l, k-l, wl, uref, hpc_g, rref, qstr, tu, exz, e_rate, ql, rid);
cigar_gen_by_chain_adv(z, cl, cl->length, ch_n, aln->a+l, k-l, wl, uref, hpc_g, rref, qstr, tu, exz, exz1, e_rate, ql, rid);
cigar_gen_by_chain_adv(z, ch_a, ch_n, &(cl->chainDP), aln->a+l, k-l, wl, uref, hpc_g, rref, qstr, tu, exz, aux_o, e_rate, ql, rid);
// m = fusion_coordinates(z, ch_a, ch_n, aln->a+l, k-l);
}
// m += cigar_gen_cns(z, cl, aln->a+l, k-l, i, wl, uref, hpc_g, rref, qstr, tu, exz, e_rate, ql, rid, aln->a+m);
@@ -14453,9 +14487,10 @@ kv_ul_ov_t *aln, uint64_t rid, int64_t max_lgap, double sgap_rate)
}
void ul_lalign(overlap_region_alloc* ol, Candidates_list *cl, const ul_idx_t *uref, char *qstr,
uint64_t ql, UC_Read* qu, UC_Read* tu, Correct_dumy* dumy, bit_extz_t *exz,
bit_extz_t *exz1, haplotype_evdience_alloc* hap, kvec_t_u64_warp* v_idx,
haplotype_evdience_alloc* hap, kvec_t_u64_warp* v_idx, overlap_region *aux_o,
double e_rate, int64_t wl, kv_ul_ov_t *aln, int64_t sid, uint64_t khit, void *km)
{
uint64_t i, bs, k, ovl/**, on**/; Window_Pool w; double err;
@@ -14511,7 +14546,7 @@ void ul_lalign(overlap_region_alloc* ol, Candidates_list *cl, const ul_idx_t *ur
///coordinates for all intervals with cov > 1
copy_asg_arr(iidx, hap->snp_srt); copy_asg_arr(buf, v_idx->a);
// fprintf(stderr, "\n[M::%s] iidx_n::%ld\n", __func__, (int64_t)iidx.n);
ul_gap_filling_adv(ol, cl, aln, wl, uref, NULL, NULL, qu->seq, tu, exz, exz1, &buf, &iidx, err, ql, sid, khit, 1, MAX_LGAP(ql));
ul_gap_filling_adv(ol, cl, aln, wl, uref, NULL, NULL, qu->seq, tu, exz, aux_o, &buf, &iidx, err, ql, sid, khit, 1, MAX_LGAP(ql));
copy_asg_arr(hap->snp_srt, iidx); copy_asg_arr(v_idx->a, buf);
// for (i = 0; (i < ol->length) && (ol->list[i].is_match == 1); i++); on = i;
// if(on <= 1) return;
Correct.h
+1 -1
View File
@@ -1136,7 +1136,7 @@ void correct_ul_overlap(overlap_region_alloc* overlap_list, const ul_idx_t *uref
double max_ov_diff_ec, long long winLen, void *km);
void ul_lalign(overlap_region_alloc* ol, Candidates_list *cl, const ul_idx_t *uref, char *qstr,
uint64_t ql, UC_Read* qu, UC_Read* tu, Correct_dumy* dumy, bit_extz_t *exz,
bit_extz_t *exz1, haplotype_evdience_alloc* hap, kvec_t_u64_warp* v_idx,
haplotype_evdience_alloc* hap, kvec_t_u64_warp* v_idx, overlap_region *aux_o,
double e_rate, int64_t wl, kv_ul_ov_t *aln, int64_t sid, uint64_t hpc_k, void *km);
void ul_lalign_old_ed(overlap_region_alloc* ol, Candidates_list *cl, const ul_idx_t *uref, char *qstr,
inter.cpp
+18 -4
View File
@@ -6030,7 +6030,19 @@ static void worker_for_ul_scall_alignment(void *data, long i, int tid) // callba
// fprintf(stderr, "[M::%s::%lu::] l1->%u; l2->%u\n", UL_INF.nid.a[s->id+i].a, s->len[i], l1, l2);
}
overlap_region *gen_aux_ovlp(overlap_region_alloc* ol)
{
if (ol->length + 1 > ol->size) {
uint64_t sl = ol->size;
ol->size = ol->length + 1;
kroundup64(ol->size);
REALLOC(ol->list, ol->size);
/// need to set new space to be 0
memset(ol->list + sl, 0, sizeof(overlap_region)*(ol->size - sl));
}
memset((&(ol->list[ol->length+1])), 0, sizeof(overlap_region));
return &(ol->list[ol->length+1]);
}
static void worker_for_ul_rescall_alignment(void *data, long i, int tid) // callback for kt_for()
{
@@ -6040,6 +6052,7 @@ static void worker_for_ul_rescall_alignment(void *data, long i, int tid) // call
int64_t /**rid = s->id+i,**/ winLen = MIN((((double)THRESHOLD_MAX_SIZE)/s->opt->diff_ec_ul), WINDOW), ton = 0;
uint32_t high_occ = 2, phase = 1;
asg64_v b0, b1, b2;
overlap_region *aux_o = NULL;
// uint64_t align = 0;
// if(UL_INF.a[s->id+i].rlen != s->len[i]) {
@@ -6050,7 +6063,7 @@ static void worker_for_ul_rescall_alignment(void *data, long i, int tid) // call
// if(s->id+i!=41927 && s->id+i!=47072 && s->id+i!=67641 && s->id+i!=90305 && s->id+i!=698342 && s->id+i!=329421) {
// return;
// }
// if((s->id+i!=291) /**&& (s->id+i!=44) && (s->id+i!=948)**/) return;
// if((s->id+i!=6) /**&& (s->id+i!=44) && (s->id+i!=948)**/) return;
// fprintf(stderr, "\n[M::%s] rid::%ld, len::%lu, name::%.*s\n", __func__, s->id+i, s->len[i],
// (int32_t)UL_INF.nid.a[s->id+i].n, UL_INF.nid.a[s->id+i].a);
@@ -6073,10 +6086,11 @@ static void worker_for_ul_rescall_alignment(void *data, long i, int tid) // call
// memset(&b->self_read, 0, sizeof(b->self_read));
ul_lalign(&b->olist, &b->clist, s->uu, s->seq[i], s->len[i], &b->self_read, &b->ovlp_read,
&b->correct, &b->exz, &b->exz1, &b->hap, &b->r_buf, s->opt->diff_ec_ul, winLen, NULL, s->id+i, s->opt->k, NULL);
&b->correct, &b->exz, &b->hap, &b->r_buf, aux_o, s->opt->diff_ec_ul, winLen, NULL, s->id+i, s->opt->k, NULL);
// ul_lalign_old_ed(&b->olist, &b->clist, s->uu, s->seq[i], s->len[i], &b->self_read, &b->ovlp_read,
// &b->correct, &b->hap, &b->r_buf, s->opt->diff_ec_ul, winLen, 1, NULL);
ton = b->olist.length;//all alignments pass similary check
aux_o = gen_aux_ovlp(&b->olist);///must be here
gl_chain_flter(&b->olist, &b->correct, &(s->sps[tid]), bl, s->uu, s->opt->diff_ec_ul, winLen, s->len[i], s->uopt, &phase);
if(phase) {
@@ -6092,7 +6106,7 @@ static void worker_for_ul_rescall_alignment(void *data, long i, int tid) // call
copy_asg_arr(b->hap.snp_srt, b0); copy_asg_arr(s->sps[tid], b1); copy_asg_arr(b->r_buf.a, b2);
ul_lalign(&b->olist, &b->clist, s->uu, s->seq[i], s->len[i], &b->self_read, &b->ovlp_read,
&b->correct, &b->exz, &b->exz1, &b->hap, &b->r_buf, s->opt->diff_ec_ul, winLen, &(bl->lo), s->id+i, s->opt->k, NULL);
&b->correct, &b->exz, &b->hap, &b->r_buf, aux_o, s->opt->diff_ec_ul, winLen, &(bl->lo), s->id+i, s->opt->k, NULL);
// ul_lalign_old_ed(&b->olist, &b->clist, s->uu, s->seq[i], s->len[i], &b->self_read, &b->ovlp_read,
// &b->correct, &b->hap, &b->r_buf, s->opt->diff_ec_ul, winLen, 0, NULL);
}