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minimap2.1
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minimap2.1
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.TH minimap2 1 "2 April 2025" "minimap2-2.28-dirty (r1248)" "Bioinformatics tools"
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.TH minimap2 1 "6 April 2025" "minimap2-2.28-dirty (r1259)" "Bioinformatics tools"
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.SH NAME
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.PP
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minimap2 - mapping and alignment between collections of DNA sequences
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@@ -418,7 +418,7 @@ Penalty of a mismatch involving ambiguous bases [1].
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.TP
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.BI --pe-ind-chain
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For paired-end short reads, perform chaining for each end independently.
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By default, minimap2 chains the two ends together.
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By default, minimap2 jointly chains the two ends.
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.TP
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.BR --splice-flank = yes | no
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Assume the next base to a
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.BR --junc-bed .
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.TP
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.BR --junc-bed \ FILE
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Gene annotations in the BED12 format (aka 12-column BED), or intron positions
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in 5-column BED. With this option, minimap2 prefers splicing in annotations.
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BED12 file can be converted from GTF/GFF3 with `paftools.js gff2bed anno.gtf'
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[].
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Junctions to prefer during base alignment.
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.I FILE
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can be gene annotations in the BED12 format (aka 12-column BED), or intron
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positions in 5-column BED. BED12 file can be converted from GTF/GFF3 with
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`paftools.js gff2bed anno.gtf'. It is
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.I NOT
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recommended to apply this option for short RNA-seq reads. []
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.TP
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.BR --junc-bonus \ INT
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Score bonus for a splice donor or acceptor found in annotation [9]. Effective with
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@@ -464,6 +467,13 @@ Score bonus for a splice donor or acceptor found in annotation [9]. Effective wi
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but not
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.BR --spsc .
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.TP
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.BR --jump-bed \ FILE
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Junctions used to extend alignment towards ends of reads. Same format as with
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.BR --junc-bed .
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This option is intended for short RNA-seq reads, while
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.B --junc-bed
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for long noisy RNA-seq reads. []
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.TP
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.BI --end-seed-pen \ INT
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Drop a terminal anchor if
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.IR s <log( g )+ INT ,
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